Batch and continuous hydrolysis of lactose using β-galactosidase immobilized on gelatin-CMC

被引:9
作者
Sungur, S [1 ]
Yildirim, Ö
机构
[1] Ankara Univ, Fac Sci, Dept Chem, TR-06100 Ankara, Turkey
[2] Ankara Univ, Fac Sci, Dept Biol, TR-06100 Ankara, Turkey
关键词
D O I
10.1080/03602559909351616
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
beta-Galactosidase enzyme (E,C. 3.2.1.23) was immobilized onto a gelatin-carboxymethylcellulose carrier system by using cross-linker chromium acetate, A relative activity increase of 33% was achieved by adding carboxymethylcellulose to gelatin, The effect of operation temperature and pH on relative activity and reusability of the immobilized enzyme was investigated, A fluidized-bed reactor with a downflow mechanism was used for continuous process hydrolysis, and a constant glucose production rate was obtained.
引用
收藏
页码:821 / 829
页数:9
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