IMMOBILIZATION OF BETA-GALACTOSIDASE ONTO GELATIN BY GLUTARALDEHYDE AND CHROMIUM(III) ACETATE

被引：36

作者：

SUNGUR, S ^{[1
]}

AKBULUT, U ^{[1
]}

机构：

[1] MIDDLE E TECH UNIV,DEPT CHEM,06531 ANKARA,TURKEY

来源：

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY | 1994年 / 59卷 / 03期

关键词：

IMMOBILIZATION OF BETA-GALACTOSIDASE; HYDROLYSIS OF LACTOSE; CROSS-LINKED GELATIN; CHROMIUM(III) ACETATE CROSS-LINKER; GLUTARALDEHYDE CROSS-LINKER;

D O I：

10.1002/jctb.280590314

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Beta-Galactosidase (EC 3.2.1.23) was immobilised onto a gelatin carrier system by crosslinking. Glutaraldehyde and chromium(III) acetate were used as crosslinking agents. The effect of pH on enzyme activity, kinetic parameters and reusability of the immobilised enzyme were investigated. To obtain a better relative activity, a method was developed and further modified to increase the surface area of the support system by using polyacrylamide. Relative activity enhancement of 68% was achieved in the gelatin-glutaraldehyde system.

引用

页码：303 / 306

页数：4

共 14 条

[1]

AXELSSON A, 1991, J CHEM TECHNOL BIOT, V52, P227

[2]

BAKKEN AB, 1992, BIOTECHNOL BIOENG, V39, P409

[3] A NEW METHOD FOR REVERSIBLE IMMOBILIZATION OF THIOL BIOMOLECULES BASED ON SOLID-PHASE BOUND THIOLSULFONATE GROUPS [J].