A 51 kDa protein kinase of potato activated with hyphal wall components from Phytophthora infestans

被引:13
作者
Katou, S [1 ]
Senda, K [1 ]
Yoshioka, H [1 ]
Doke, N [1 ]
Kawakita, K [1 ]
机构
[1] Nagoya Univ, Grad Sch Bioagr Sci, Plant Pathol Lab, Chikusa Ku, Nagoya, Aichi 4648601, Japan
关键词
elicitor; hyphal wall components; potato; protein kinase; signal transduction;
D O I
10.1093/oxfordjournals.pcp.a029611
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In potato tuber tissue, treatment of fungal elicitor, hyphal wall components (HWC) induces various plant defense reactions. As treatment of protein kinase inhibitor prior to HWC treatment blocks some of defense reactions induced by HWC, involvement of protein kinases in plant defense induction is proposed. Here, we demonstrate HWC-induced activation of a 51-kDa protein kinase (abbreviated p51-PK) using myelin basic protein as a substrate in potato tuber discs. The activity of pS1-PK was not detected in the absence of phosphatase inhibitor, NaF, and p51-PK was immunoprecipitated with antibody against phosphotyrosine. Pretreatment of phospholipase C inhibitor, neomycin, and GTP-binding protein activator, mastoparan, partially inhibited the HWC-induced activation of p51-PK, suggesting possible involvement of phospholipase C and GTP-binding protein in the activation of p51-PK. Exogenously supplied elicitors, salicylic acid and arachidonic acid, which are known to induce various defense responses in potato plants, also activated the protein kinase showing the same migration as p51-PK on SDS-PAGE and different activation patterns. These results implied that p51-PK might be involved in several signal transduction pathways leading to plant defense responses initiated by different stimuli.
引用
收藏
页码:825 / 831
页数:7
相关论文
共 37 条
[21]   Environmental stress response in plants: The role of mitogen-activated protein kinases [J].
Mizoguchi, T ;
Ichimura, K ;
Shinozaki, K .
TRENDS IN BIOTECHNOLOGY, 1997, 15 (01) :15-19
[22]  
OBA K, 1985, PLANT CELL PHYSIOL, V26, P873
[23]  
Park HJ, 1998, PLANT CELL PHYSIOL, V39, P1218, DOI 10.1093/oxfordjournals.pcp.a029323
[24]   Rapid Avr9- and Cf-9-dependent activation of MAP kinases in tobacco cell cultures and leaves: Convergence of resistance gene, elicitor, wound, and salicylate responses [J].
Romeis, T ;
Piedras, P ;
Zhang, SQ ;
Klessig, DF ;
Hirt, H ;
Jones, JDG .
PLANT CELL, 1999, 11 (02) :273-287
[25]   PROTEIN KINASES .7. THE MAPK SIGNALING CASCADE [J].
SEGER, R ;
KREBS, EG .
FASEB JOURNAL, 1995, 9 (09) :726-735
[26]   Effect of mastoparan on phospholipase A2 activity in potato tubers treated with fungal elicitor [J].
Senda, K ;
Doke, N ;
Kawakita, K .
PLANT AND CELL PHYSIOLOGY, 1998, 39 (10) :1080-1086
[27]   Jasmonate-based wound signal transduction requires activation of WIPK, a tobacco mitogen-activated protein kinase [J].
Seo, S ;
Sano, H ;
Ohashi, Y .
PLANT CELL, 1999, 11 (02) :289-298
[28]   TOBACCO MAP KINASE - A POSSIBLE MEDIATOR IN WOUND SIGNAL-TRANSDUCTION PATHWAYS [J].
SEO, S ;
OKAMOTO, N ;
SETO, H ;
ISHIZUKA, K ;
SANO, H ;
OHASHI, Y .
SCIENCE, 1995, 270 (5244) :1988-1992
[29]  
Subramaniam R, 1997, PLANT CELL, V9, P653, DOI 10.1105/tpc.9.4.653
[30]   Slow and prolonged activation of the p47 protein kinase during hypersensitive cell death in a culture of tobacco cells [J].
Suzuki, K ;
Yano, A ;
Shinshi, H .
PLANT PHYSIOLOGY, 1999, 119 (04) :1465-1472