Limited intercellular spread of spontaneous Ca2+ signals via gap junctions between mouse chromaffin cells in situ

被引：6

作者：

Yamagami, K ^{[1
]}

Moritoyo, T ^{[1
]}

Wakamori, M ^{[1
]}

Sorimachi, M ^{[1
]}

机构：

[1] Kagoshima Univ, Fac Med, Dept Physiol, Kagoshima 8908520, Japan

来源：

NEUROSCIENCE LETTERS | 2002年 / 323卷 / 02期

关键词：

confocal laser-scanning microscope; oregon green BAPTA-1/AM; fura-2; microfluorometry; the cytosolic Ca2+ concentration; mouse adrenal chromaffin cells; gap junctions; ATP;

D O I：

10.1016/S0304-3940(01)02578-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Using a confocal laser-scanning microscope, we measured the changes in the cytosolic Ca2+, concentration ([Ca](i)) of chromaffin cells on adrenal slice preparations of mouse. The spontaneous fluctuations of [Ca](i) were often observed in situ, as reported in isolated rat and bovine cells. Intriguingly, the spontaneous [Call changes in one cell were often transmitted to one or two adjacent cells, and the synchronized [Ca](i) changes were often observed in two adjacent cells, both of which failed to respond to ATP. The synchronized [Ca](i) changes between two cells were much less frequently observed in rat than in mouse adrenals. The spontaneous [Ca](i) changes were also synchronized between closely touching mouse chromaffin cells in culture. These results suggest that the spread of Ca 21 signaling is mediated by low-conductance intercellular coupling. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：97 / 100

页数：4

共 20 条

[11]

HOLLMAN ME, 1994, J PHYSIOL-LONDON, V478, P115

[12] SYNAPTIC ACTIVATION OF RAT ADRENAL-MEDULLA EXAMINED WITH A LARGE PHOTODIODE ARRAY IN COMBINATION WITH A VOLTAGE-SENSITIVE DYE [J].

IIJIMA, T ;

MATSUMOTO, G ;

KIDOKORO, Y .

NEUROSCIENCE, 1992, 51 (01) :211-219

[13] Influence of cell number on the characteristics and synchrony of Ca2+ oscillations in clusters of mouse pancreatic islet cells [J].

Jonkers, FC ;

Jonas, JC ;

Gilon, P ;

Henquin, JC .

JOURNAL OF PHYSIOLOGY-LONDON, 1999, 520 (03) :839-849

[14] Functional organization of chromaffin cells and cholinergic synaptic transmission in rat adrenal medulla [J].

Kajiwara, R ;

Sand, O ;

Kidokoro, Y ;

Barish, ME ;

Iijima, T .

JAPANESE JOURNAL OF PHYSIOLOGY, 1997, 47 (05) :449-464

[15] CELLULAR-RESPONSES TO CA-2+ FROM EXTRACELLULAR AND INTRACELLULAR SOURCES ARE DIFFERENT AS SHOWN BY SIMULTANEOUS MEASUREMENTS OF CYTOSOLIC CA-2+ AND SECRETION FROM BOVINE CHROMAFFIN CELLS [J].

KIM, KT ;

WESTHEAD, EW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (24) :9881-9885

[16] Low-conductance intercellular coupling between mouse chromaffin cells in situ [J].

Moser, T .

JOURNAL OF PHYSIOLOGY-LONDON, 1998, 506 (01) :195-205

[17] HEPATOCYTE GAP-JUNCTIONS ARE PERMEABLE TO THE 2ND MESSENGER, INOSITOL 1,4,5-TRISPHOSPHATE, AND TO CALCIUM-IONS [J].

SAEZ, JC ;

CONNOR, JA ;

SPRAY, DC ;

BENNETT, MVL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (08) :2708-2712

[18] STIMULATION BY ATP OF INOSITOL TRISPHOSPHATE ACCUMULATION AND CALCIUM MOBILIZATION IN CULTURED ADRENAL CHROMAFFIN CELLS [J].

SASAKAWA, N ;

NAKAKI, T ;

YAMAMOTO, S ;

KATO, R .

JOURNAL OF NEUROCHEMISTRY, 1989, 52 (02) :441-447

[19] SPONTANEOUS AND SECRETAGOGUE-INDUCED CHANGES IN CYTOSOLIC FREE CA CONCENTRATION MEASURED BY MICROFLUORIMETRY WITH FURA-2 ON SINGLE BOVINE ADRENAL CHROMAFFIN CELLS [J].

SORIMACHI, M ;

YAMAGAMI, K ;

YADA, T ;

NISHIMURA, S .

JAPANESE JOURNAL OF PHYSIOLOGY, 1989, 39 (05) :687-701

[20]

STAUFFER PL, 1993, J BIOL CHEM, V268, P19769

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