Determination of tetramethylenedisulfotetramine in human urine with gas chromatograph-flame thermionic detection coupling with direct immersed solid-phase micro-extraction

被引：23

作者：

Zeng, Dong

Chen, Bo ^{[1
]}

Yao, Shouzhuo

Ying, Jianying

机构：

[1] Hunan Normal Univ, Minist Educ, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Peoples R China

[2] Hunan Publ Secur Dept, Changsha 410001, Peoples R China

来源：

FORENSIC SCIENCE INTERNATIONAL | 2006年 / 159卷 / 2-3期

关键词：

tetramethylenedisulfotetramine; gas chromatography; solid-phase micro-extraction; urine;

D O I：

10.1016/j.forsciint.2005.07.013

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

An analytical method for determination of tetramethylenedisulfotetramine (tetramine) in human urine by gas chromatography-flame thermionic detection (GC-FTD) coupling with a direct immersed solid-phase micro-extraction (DI-SPME) was developed. The enrichment effects of three fiber coatings of SPME for tetramine were compared. Results showed that the enrichment effect of polar 85 mu m polyacrylate (PA) coating was better than that of apolar 100 and 7 mu m polydimethylsiloxane (PDMS) coatings. Other experimental parameters, such as ionic strength, volume, temperature of sample solution and time for extraction, time and temperature for desorption, were also optimized. The correlation coefficient of the calibration curve was 0.9998 in the range of 0.082-.1.0 ng/mL for tetramine. The limit of quantitation of tetramine in urine was 0.082 ng/mL. In this method, the sample pretreatment is simple and convenient. As a monitoring means, it has been successfully applied to detection of tetramine toxicosis in criminal cases, as well as clinical therapy of poisoned sufferer. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

引用

页码：168 / 174

页数：7

共 23 条

[1] SIMULTANEOUS IDENTIFICATION AND DETERMINATION OF TETRAMINE IN MARINE SNAILS BY PROTON NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY [J].

ANTHONI, U ;

CHRISTOPHERSEN, C ;

NIELSEN, PH .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1989, 37 (03) :705-707

[2]

ARHUR CL, 1990, ANAL CHEM, V62, P2145

[3] Quantitative and qualitative analysis of MDMA, MDEA, MA and amphetamine in urine by head-space/solid phase micro-extraction (SPME) and GC/MS [J].

Centini, F ;

Masti, A ;

Comparini, IB .

FORENSIC SCIENCE INTERNATIONAL, 1996, 83 (03) :161-166

[4] Simultaneous detection of some drugs of abuse in saliva samples by SPME technique [J].

Fucci, N ;

De Giovanni, N ;

Chiarotti, M .

FORENSIC SCIENCE INTERNATIONAL, 2003, 134 (01) :40-45

[5]

GAO L, 2002, CHIN J HLTH LAB TECH, V12, P438

[6]

GONG ZP, 1999, CHIN J INSTRUM ANAL, V18, P70

[7]

GUAN FY, 1993, CHINESE J CHROMATOGR, V11, P333

[8]

GUAN FY, 1993, CHINESE J ANAL CHEM, V21, P452

[9] Simultaneous determination of barbiturates in human biological fluids by direct immersion solid-phase microextraction and gas chromatography-mass spectrometry [J].

Iwai, M ;

Hattori, H ;

Arinobu, T ;

Ishii, A ;

Kumazawa, T ;

Noguchi, H ;

Suzuki, O ;

Seno, H .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2004, 806 (01) :65-73

[10] DETERMINATION OF TETRAMINE IN POSTMORTEM SPECIMENS BY GC-NPD [J].

JING, S ;

YU, ZS ;

ZHAO, LZ ;

ZHOU, HZ .

JOURNAL OF ANALYTICAL TOXICOLOGY, 1994, 18 (05) :275-277

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