Expansion of Multipotent Stem Cells from the Adult Human Brain

被引：35

作者：

Murrell, Wayne ^{[1
,6
]}

Palmero, Emily ^{[1
]}

Bianco, John ^{[1
]}

Stangeland, Biljana ^{[1
]}

Joel, Mrinal ^{[1
]}

Paulson, Linda ^{[1
]}

Thiede, Bernd ^{[2
]}

Grieg, Zanina ^{[1
]}

Ramsnes, Ingunn ^{[1
]}

Skjellegrind, Havard K. ^{[1
]}

Nygard, Stale ^{[3
]}

Brandal, Petter ^{[4
]}

Sandberg, Cecilie ^{[1
]}

Vik-Mo, Einar ^{[1
]}

Palmero, Sheryl ^{[1
]}

Langmoen, Iver A. ^{[1
,5
,6
]}

机构：

[1] Oslo Univ Hosp, Inst Surg Res, Vilhelm Magnus Lab Neurosurg Res, Oslo, Norway

[2] Univ Oslo, Biotechnol Ctr Oslo, Oslo, Norway

[3] Univ Oslo, Res Grp Biomed Informat, Oslo, Norway

[4] Norwegian Radium Hosp, Ctr Canc Biomed, Oslo, Norway

[5] Oslo Univ Hosp, Dept Neurosurg, Oslo, Norway

[6] Oslo Univ Hosp, Norwegian Ctr Stem Cell Res, Oslo, Norway

来源：

PLOS ONE | 2013年 / 8卷 / 08期

关键词：

PROGENITOR CELLS; GENE-EXPRESSION; CHROMOSOME-ABNORMALITIES; NEURAL PROGENITORS; IPS CELLS; TRANSPLANTATION; NEURONS; DIFFERENTIATION; DISEASE; DEVELOP;

D O I：

10.1371/journal.pone.0071334

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The discovery of stem cells in the adult human brain has revealed new possible scenarios for treatment of the sick or injured brain. Both clinical use of and preclinical research on human adult neural stem cells have, however, been seriously hampered by the fact that it has been impossible to passage these cells more than a very few times and with little expansion of cell numbers. Having explored a number of alternative culturing conditions we here present an efficient method for the establishment and propagation of human brain stem cells from whatever brain tissue samples we have tried. We describe virtually unlimited expansion of an authentic stem cell phenotype. Pluripotency proteins Sox2 and Oct4 are expressed without artificial induction. For the first time multipotency of adult human brain-derived stem cells is demonstrated beyond tissue boundaries. We characterize these cells in detail in vitro including microarray and proteomic approaches. Whilst clarification of these cells' behavior is ongoing, results so far portend well for the future repair of tissues by transplantation of an adult patient's own-derived stem cells.

引用

页数：23

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