Acquisition of iron by the non-siderophore-producing Pseudomonas fragi

被引:40
作者
ChampomierVerges, MC
Stintzi, A
Meyer, JM
机构
[1] UNIV STRASBOURG 1,CNRS,URA D1481,LAB MICROBIOL & GENET,F-67083 STRASBOURG,FRANCE
[2] INRA,LAB RECH VIANDE,F-78352 JOUY EN JOSAS,FRANCE
来源
MICROBIOLOGY-UK | 1996年 / 142卷
关键词
iron acquisition; Pseudomonas fragi; siderophore;
D O I
10.1099/13500872-142-5-1191
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The iron requirement, siderophore production and iron uptake mechanism of the type strain Pseudomonas fragi ATCC 4973 and five P. fragi isolates from meat were analysed. The strains exhibited a high sensitivity to iron starvation: their growth was strongly inhibited in medium supplemented with the iron chelator ethylenediamine di(hydroxyphenylacetic acid) or in medium treated with 8-hydroxyguinoline to remove contaminating iron. No siderophores were detectable in the growth supernatants of iron-starved cells. Cross-feeding experiments in iron-depleted medium showed, however, that the bacterial growth could be strongly stimulated by siderophores of foreign origin including desferriferrioxamine B, enterobactin and some pyoverdines. Moreover, all the strains were capable of efficiently using the iron sources present in their natural environment, i.e. transferrin, lactoferrin and haemoglobin. Iron starvation led to the specific production of supplementary outer-membrane proteins of apparent molecular mass ranging from 80 to 88 kDa. Furthermore, growth in the presence of exogenous siderophores resulted, in some strains, in the induction of siderophore-mediated iron uptake systems. For one strain the concomitant synthesis of an iron-regulated, siderophore-inducible outer-membrane protein was observed.
引用
收藏
页码:1191 / 1199
页数:9
相关论文
共 68 条
[1]   IRON ACQUISITION-SYSTEMS OF LISTERIA-MONOCYTOGENES [J].
ADAMS, TJ ;
VARTIVARIAN, S ;
COWART, RE .
INFECTION AND IMMUNITY, 1990, 58 (08) :2715-2718
[2]  
[Anonymous], [No title captured]
[3]   LACTOBACILLUS-PLANTARUM, AN ORGANISM NOT REQUIRING IRON [J].
ARCHIBALD, F .
FEMS MICROBIOLOGY LETTERS, 1983, 19 (01) :29-32
[4]   MOLECULAR MECHANISM OF REGULATION OF SIDEROPHORE-MEDIATED IRON ASSIMILATION [J].
BAGG, A ;
NEILANDS, JB .
MICROBIOLOGICAL REVIEWS, 1987, 51 (04) :509-518
[5]   IDENTIFICATION AND CHARACTERIZATION OF THE EXBB, EXBD AND TONB GENES OF PSEUDOMONAS-PUTIDA WCS358 - THEIR INVOLVEMENT IN FERRIC-PSEUDOBACTIN TRANSPORT [J].
BITTER, W ;
TOMMASSEN, J ;
WEISBEEK, PJ .
MOLECULAR MICROBIOLOGY, 1993, 7 (01) :117-130
[6]  
BRAUN V, 1987, IRON TRANSPORT MICRO, P35
[7]   SECONDARY METABOLITES FROM FLUORESCENT PSEUDOMONADS [J].
BUDZIKIEWICZ, H .
FEMS MICROBIOLOGY LETTERS, 1993, 104 (3-4) :209-228
[8]   ANTIBACTERIAL ACTIVITY AMONG PSEUDOMONAS STRAINS OF MEAT ORIGIN [J].
CHAMPOMIERVERGES, MC ;
RICHARD, J .
LETTERS IN APPLIED MICROBIOLOGY, 1994, 18 (01) :18-20
[9]   CHARACTERIZATION OF PYOVERDINPSS, THE FLUORESCENT SIDEROPHORE PRODUCED BY PSEUDOMONAS-SYRINGAE PV SYRINGAE [J].
CODY, YS ;
GROSS, DC .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (05) :928-934
[10]   EVIDENCE FOR DIFFERENT PYOVERDINE-MEDIATED IRON UPTAKE SYSTEMS AMONG PSEUDOMONAS-AERUGINOSA STRAINS [J].
CORNELIS, P ;
HOHNADEL, D ;
MEYER, JM .
INFECTION AND IMMUNITY, 1989, 57 (11) :3491-3497