Hydrolysis of acid and alkali presoaked lignocellulosic biomass exposed to electron beam irradiation

被引:30
作者
Karthika, K. [1 ]
Arun, A. B. [1 ]
Melo, J. S. [2 ]
Mittal, K. C. [3 ]
Kumar, Mukesh [3 ]
Rekha, P. D. [1 ]
机构
[1] Yenepoya Univ, Yenepoya Res Ctr, Mangalore 575018, Karnataka, India
[2] Bhabha Atom Res Ctr, Nucl Agr & Biotechnol Div, Bombay 400085, Maharashtra, India
[3] BARC, BTD Grp, Electron Beam Ctr, Accelerator & Pulse Power Div, Bombay 400085, Maharashtra, India
关键词
Enzymatic hydrolysis; Alkali soaking; Acid soaking; Electron beam irradiation; Lignocellulosic biomass; CELLULOSE CRYSTALLINE-STRUCTURE; ENZYMATIC-HYDROLYSIS; RICE STRAW; PRETREATMENT;
D O I
10.1016/j.biortech.2012.12.048
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
In this study, synergetic effect of mild acid and alkali with electron beam irradiation (EBI) on the enzymatic hydrolysis of a selected grass biomass was assessed. Biomass samples prepared by soaking with 1% H2SO4, or 1% NaOH, were exposed to 75 and 150 kGy of EBI. Water presoaked biomass was used as control. Hydrolysis of pretreated samples was carried out using cellulase (15 FPU/g biomass) for 120 h. Structural changes were studied by FTIR and XRD analyses. Reducing sugar and glucose yields from enzymatic hydrolysis were significantly higher in acid and alkali presoaked EBI exposed samples. Theoretical glucose yield showed 40% increase from control in alkali presoaked EBI exposed (150 kGy) samples. Removal of hemicellulose, decreased crystallinity and structural changes were major factors for the combined treatment effect favoring the hydrolysis. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:646 / 649
页数:4
相关论文
共 20 条
[1]   Improved enzymatic hydrolysis yield of rice straw using electron beam irradiation pretreatment [J].
Bak, Jin Seop ;
Ko, Ja Kyong ;
Han, Young Hwan ;
Lee, Byung Cheol ;
Choi, In-Geol ;
Kim, Kyoung Heon .
BIORESOURCE TECHNOLOGY, 2009, 100 (03) :1285-1290
[2]   Transition of Cellulose Crystalline Structure and Surface Morphology of Biomass as a Function of Ionic Liquid Pretreatment and Its Relation to Enzymatic Hydrolysis [J].
Cheng, Gang ;
Varanasi, Patanjali ;
Li, Chenlin ;
Liu, Hanbin ;
Menichenko, Yuri B. ;
Simmons, Blake A. ;
Kent, Michael S. ;
Singh, Seema .
BIOMACROMOLECULES, 2011, 12 (04) :933-941
[3]  
Ershov B.G., 1998, Russian Chemical Reviews, V67, P315, DOI [10.1070/RC1998v067n04ABEH000379, DOI 10.1070/RC1998V067N04ABEH000379]
[4]   RELATIVE SUSCEPTIBILITY OF FORAGES TO HEAT DAMAGE AS AFFECTED BY MOISTURE, TEMPERATURE, AND PH [J].
GOERING, HK ;
VANSOEST, PJ ;
HEMKEN, RW .
JOURNAL OF DAIRY SCIENCE, 1973, 56 (01) :137-143
[5]   Pretreatments to enhance the digestibility of lignocellulosic biomass [J].
Hendriks, A. T. W. M. ;
Zeeman, G. .
BIORESOURCE TECHNOLOGY, 2009, 100 (01) :10-18
[6]   Enzymatic hydrolysis and characterization of lignocellulosic biomass exposed to electron beam irradiation [J].
Karthika, K. ;
Arun, A. B. ;
Rekha, P. D. .
CARBOHYDRATE POLYMERS, 2012, 90 (02) :1038-1045
[7]   FT-IR microscopic analysis of changing cellulose crystalline structure during wood cell wall formation [J].
Kataoka, Y ;
Kondo, T .
MACROMOLECULES, 1998, 31 (03) :760-764
[8]   Methods for Pretreatment of Lignocellulosic Biomass for Efficient Hydrolysis and Biofuel Production [J].
Kumar, Parveen ;
Barrett, Diane M. ;
Delwiche, Michael J. ;
Stroeve, Pieter .
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 2009, 48 (08) :3713-3729
[9]   USE OF DINITROSALICYLIC ACID REAGENT FOR DETERMINATION OF REDUCING SUGAR [J].
MILLER, GL .
ANALYTICAL CHEMISTRY, 1959, 31 (03) :426-428
[10]  
NREL, 2008, STAND BIOM AN PROC