Detection of rifampin resistance in Mycobacterium tuberculosis by use of a rapid, simple, and specific RNA/RNA mismatch assay

被引：40

作者：

Nash, KA ^{[1
]}

Gaytan, A ^{[1
]}

Inderlied, CB ^{[1
]}

机构：

[1] PK CLIN LABS,LOS ANGELES,CA

来源：

JOURNAL OF INFECTIOUS DISEASES | 1997年 / 176卷 / 02期

关键词：

D O I：

10.1086/517283

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

An adaption of an RNA/RNA duplex, base pair-mismatch assay is capable of detecting rifampin resistance in Mycobacterium tuberculosis, The specificity and sensitivity of the mismatch assay in detecting rifampin resistance were 100% and 96%, respectively, when tested against 46 rifampin-resistant and rifampin-susceptible strains of M. tuberculosis, By use of a range of mycobacterial and nonmycobacterial prokaryote pathogens, the mismatch assay was shown to be specific for M. tuberculosis and Mycobacterium bovis. The assay is cost-effective compared with DNA sequencing and other molecular methods and is simple to perform and interpret, Furthermore, the assay can return a result within 24 h after receipt of an isolated organism and potentially can be used directly with smear-positive specimens.

引用

页码：533 / 536

页数：4

共 14 条

[1] RAPID DETECTION OF RIFAMPICIN RESISTANCE IN SPUTUM AND BIOPSY SPECIMENS FROM TUBERCULOSIS PATIENTS BY PCR AND LINE PROBE ASSAY [J].