Analysis of the sequence of Dioscorea alata bacilliform virus;: Comparison to other members of the badnavirus group

被引:38
作者
Briddon, RW
Phillips, S
Brunt, A
Hull, R
机构
[1] John Innes Ctr Plant Sci Res, Dept Virus Res, Norwich NR4 7UH, Norfolk, England
[2] Hort Res Int, Warwick CV35 9EF, England
关键词
badnavirus; Dioscorea alata; pararetrovirus; sequence;
D O I
10.1023/A:1008076420783
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The complete nucleotide sequence of the genome of Dioscorea alata bacilliform virus (DaBV) has been determined from cloned fragments. Features of the genome confirm DaBV to be a pararetrovirus of the genus Badnavirus which is more similar to other mealy-bug transmitted badnaviruses, in particular to cacao swollen shoot virus, than to rice tungro bacilliform virus. Sequence variability between cloned fragments suggests that the genetic variability of the virus may be quite high (up to 11% nucleotide sequence variation for some small regions of the genome) although the overall variability detected was 4.2% at the nucleotide level.
引用
收藏
页码:277 / 283
页数:7
相关论文
共 26 条
[21]   CLONING BIOLOGICALLY-ACTIVE GEMINIVIRUS DNA USING PCR AND OVERLAPPING PRIMERS [J].
PATEL, VP ;
ROJAS, MR ;
PAPLOMATAS, EJ ;
GILBERTSON, RL .
NUCLEIC ACIDS RESEARCH, 1993, 21 (05) :1325-1326
[22]  
PHILLIPS S, 1998, IN PRES J PHYTOPATHO
[23]   CHARACTERIZATION OF THE GENOME OF RICE TUNGRO-BACILLIFORM VIRUS - COMPARISON WITH COMMELINA YELLOW MOTTLE VIRUS AND CAULIMOVIRUSES [J].
QU, RD ;
BHATTACHARYYA, M ;
LACO, GS ;
DEKOCHKO, A ;
RAO, BLS ;
KANIEWSKA, MB ;
ELMER, JS ;
ROCHESTER, DE ;
SMITH, CE ;
BEACHY, RN .
VIROLOGY, 1991, 185 (01) :354-364
[24]   LABELING DEOXYRIBONUCLEIC-ACID TO HIGH SPECIFIC ACTIVITY INVITRO BY NICK TRANSLATION WITH DNA-POLYMERASE I [J].
RIGBY, PWJ ;
DIECKMANN, M ;
RHODES, C ;
BERG, P .
JOURNAL OF MOLECULAR BIOLOGY, 1977, 113 (01) :237-251
[25]   DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].
SANGER, F ;
NICKLEN, S ;
COULSON, AR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467
[26]   The N-terminal portion of the 216-kDa polyprotein of Commelina yellow mottle badnavirus is required for virus movement but not for replication [J].
Tzafrir, I ;
AyalaNavarrete, L ;
Lockhart, BEL ;
Olszewski, NE .
VIROLOGY, 1997, 232 (02) :359-368