Oligonucleotide-based strategies to reduce gene expression

被引：35

作者：

Dagle, JM

Weeks, DL ^{[1
]}

机构：

[1] Univ Iowa, Dept Biochem, Iowa City, IA 52242 USA

[2] Univ Iowa, Dept Pediat, Iowa City, IA 52242 USA

来源：

DIFFERENTIATION | 2001年 / 69卷 / 2-3期

基金：

美国国家卫生研究院;

关键词：

oligonucleotides; antisense; triplex;

D O I：

10.1046/j.1432-0436.2001.690201.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Research on embryonic development and differentiation provides a sensitive, but challenging opportunity to use a variety of techniques designed to modulate gene expression. Changes in the expression of a single gene can alter levels of other genes and provide information on developmentally regulated gene expression pathways. The morphological consequences of altered gene expression can link gene expression to developmental fate. Oligonucleotide-based approaches offer a variety of means to potentially disrupt normal gene expression. The basis for some of these approaches is presented in this review.

引用

收藏

页码：75 / 82

页数：8

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共 55 条

[21] PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDES BIND TO BASIC FIBROBLAST GROWTH-FACTOR, INHIBIT ITS BINDING TO CELL-SURFACE RECEPTORS, AND REMOVE IT FROM LOW-AFFINITY BINDING-SITES OIL EXTRACELLULAR-MATRIX [J].

GUVAKOVA, MA ;

YAKUBOV, LA ;

VLODAVSKY, I ;

TONKINSON, JL ;

STEIN, CA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (06) :2620-2627

[22] INHIBITION OF KLENOW FRAGMENT DNA-POLYMERASE ON DOUBLE-HELICAL TEMPLATES BY OLIGONUCLEOTIDE-DIRECTED TRIPLE-HELIX FORMATION [J].

HACIA, JG ;

DERVAN, PB ;

WOLD, BJ .

BIOCHEMISTRY, 1994, 33 (20) :6192-6200

[23] IN-VIVO TRANSCRIPTION OF A PROGESTERONE-RESPONSIVE GENE IS SPECIFICALLY INHIBITED BY A TRIPLEX-FORMING OLIGONUCLEOTIDE [J].

ING, NH ;

BEEKMAN, JM ;

KESSLER, DJ ;

MURPHY, M ;

JAYARAMAN, K ;

ZENDEGUI, JG ;

HOGAN, ME ;

OMALLEY, BW ;

TSAI, MJ .

NUCLEIC ACIDS RESEARCH, 1993, 21 (12) :2789-2796

[24] INHIBITION OF IN-VITRO TRANSCRIPTION BY A TRIPLEX-FORMING OLIGONUCLEOTIDE TARGETED TO HUMAN C-MYC P2 PROMOTER [J].

KIM, HG ;

MILLER, DM .

BIOCHEMISTRY, 1995, 34 (25) :8165-8171

[25] Triple helix-forming oligonucleotide corresponding to the polypyrimidine sequence in the rat alpha 1(I) collagen promoter specifically inhibits factor binding and transcription [J].

Kovacs, A ;

Kandala, JC ;

Weber, KT ;

Guntaka, RV .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (03) :1805-1812

[26] Stability of stereoregular oligo(nucleoside phosphorothioate)s in human plasma: Diastereoselectivity of plasma 3'-exonuclease [J].

Koziolkiewicz, M ;

Wojcik, M ;

Kobylanska, A ;

Karwowski, B ;

Rebowska, B ;

Guga, P ;

Stec, WJ .

ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT, 1997, 7 (01) :43-48

[27] From bugs to drugs: Therapeutic immunomodulation with oligodeoxynucleotides containing CpG sequences from bacterial DNA [J].

Krieg, AM .

ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT, 2001, 11 (03) :181-188

[28] Antisense oligonucleotides: Promise and reality [J].

Lebedeva, I ;

Stein, CA .

ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 2001, 41 :403-419

[29] High-frequency intrachromosomal gene conversion induced by triplex-forming oligonucleotides microinjected into mouse cells [J].

Luo, ZJ ;

Macris, MA ;

Faruqi, AF ;

Glazer, PM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (16) :9003-9008

[30] INHIBITION OF T7 RNA-POLYMERASE INITIATION BY TRIPLE-HELICAL DNA COMPLEXES - A MODEL FOR ARTIFICIAL GENE REPRESSION [J].

MAHER, LJ .

BIOCHEMISTRY, 1992, 31 (33) :7587-7594

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