Evidence for a parathyroid hormone-2 receptor selective ligand in the hypothalamus

被引:56
作者
Usdin, TB
机构
关键词
D O I
10.1210/en.138.2.831
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The PTH2 receptor is expressed in several brain nuclei but we have been unable to detect mRNA encoding PTH, which is the only known ligand for the PTH2 receptor, in the brain. We now have evidence for a PTH2 receptor selective ligand in an acid-acetone extract made from bovine hypothalamus. The partially purified extract activates the PTH2 receptor more effectively than it activates the PTH/PTHrP receptor, while PTH activates these two receptors at similar concentration. The activity appears immunologically distinct from PTH and its effect is potently antagonized by [D-Trp(12)]bPTH(7-34). These data provide evidence for a biologically active peptide, which may be related to PTH, and which is a potential new neurotransmitter or hormone.
引用
收藏
页码:831 / 834
页数:4
相关论文
共 10 条
[1]  
ABOUSAMRA AB, 1992, CELL BIOL, V89, P2732
[2]   The human PTH2 receptor: Binding and signal transduction properties of the stably expressed recombinant receptor [J].
Behar, V ;
Pines, M ;
Nakamoto, C ;
Greenberg, Z ;
Bisello, A ;
Stueckle, SM ;
Bessalle, R ;
Usdin, TB ;
Chorev, M ;
Rosenblatt, M ;
Suva, LJ .
ENDOCRINOLOGY, 1996, 137 (07) :2748-2757
[3]  
Brooker G, 1979, Adv Cyclic Nucleotide Res, V10, P1
[4]   A NEW HIGHLY POTENT PARATHYROID-HORMONE ANTAGONIST - [D-TRP12, TYR34]BPTH-(7--34)NH2 [J].
GOLDMAN, ME ;
MCKEE, RL ;
CAULFIELD, MP ;
REAGAN, JE ;
LEVY, JJ ;
GAY, CT ;
DEHAVEN, PA ;
ROSENBLATT, M ;
CHOREV, M .
ENDOCRINOLOGY, 1988, 123 (05) :2597-2599
[5]   A G-PROTEIN LINKED RECEPTOR FOR PARATHYROID-HORMONE AND PARATHYROID-HORMONE RELATED PEPTIDE [J].
JUPPNER, H ;
ABOUSAMRA, AB ;
FREEMAN, M ;
KONG, XF ;
SCHIPANI, E ;
RICHARDS, J ;
KOLAKOWSKI, LF ;
HOCK, J ;
POTTS, JT ;
KRONENBERG, HM ;
SEGRE, GV .
SCIENCE, 1991, 254 (5034) :1024-1026
[6]  
KONIG M, 1991, Molecular and Cellular Neuroscience, V2, P331, DOI 10.1016/1044-7431(91)90063-T
[7]   IMMUNOCYTOCHEMICAL DISTRIBUTION OF PTH IMMUNOREACTIVITY IN VERTEBRATE BRAINS [J].
PANG, PKT ;
KANEKO, T ;
HARVEY, S .
AMERICAN JOURNAL OF PHYSIOLOGY, 1988, 255 (04) :R643-R647
[8]   IDENTIFICATION AND FUNCTIONAL EXPRESSION OF A RECEPTOR SELECTIVELY RECOGNIZING PARATHYROID-HORMONE, THE PTH2 RECEPTOR [J].
USDIN, TB ;
GRUBER, C ;
BONNER, TI .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (26) :15455-15458
[9]   GASTRIC-INHIBITORY POLYPEPTIDE RECEPTOR, A MEMBER OF THE SECRETIN-VASOACTIVE INTESTINAL PEPTIDE RECEPTOR FAMILY, IS WIDELY DISTRIBUTED IN PERIPHERAL ORGANS AND THE BRAIN [J].
USDIN, TB ;
MEZEY, E ;
BUTTON, DC ;
BROWNSTEIN, MJ ;
BONNER, TI .
ENDOCRINOLOGY, 1993, 133 (06) :2861-2870
[10]   Distribution of parathyroid hormone-2 receptor messenger ribonucleic acid in rat [J].
Usdin, TB ;
Bonner, TI ;
Harta, G ;
Mezey, E .
ENDOCRINOLOGY, 1996, 137 (10) :4285-4297