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Gene expression of P2X-receptors in the developing inner ear of the rat

被引：67
作者：
Brändle, U
Zenner, HP
Ruppersberg, JP
机构：
[1] Univ Tubingen, Dept Otolaryngol, D-72076 Tubingen, Germany
[2] Univ Tubingen, Inst Physiol 2, D-72076 Tubingen, Germany
来源：
NEUROSCIENCE LETTERS | 1999年 / 273卷 / 02期
关键词：
P2X-receptor; ligand-gated channel; inner ear; Organ of Corti; spiral ganglion; reverse transcription-polymerase chain reaction;
D O I：
10.1016/S0304-3940(99)00648-5
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Reverse transcription-polymerase chain reaction (RT-PCR) was used to characterize the expression of P2X receptor subunits (P2X(1)-P2X(7)) in different inner ear tissues. The present study revealed the presence of P2X(2), P2X(3), P2X(4) and P2X(7)-mRNA in rat organ of Corti, vestibular organ and spiral ganglion at different postnatal developmental stages (PD1-PD16), with slight differences in the onset of expression. Expression of P2X(1), P2X(5) and P2X(6)-mRNA was not detectable in the inner ear tissues. In addition, single cell RT-PCR experiments with outer hair cells (OHC) revealed the expression of either the P2X(2) or the P2X(2-2) splice variant or coexpression of both isoforms in individual cells. Our data suggest that extracellular adenosine-5'-triphosphate (ATP) may play an important role in signal transduction in the inner ear. (C) 1999 Elsevier Science ireland Ltd. All rights reserved.
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页码：105 / 108
页数：4
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