Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

被引：1415

作者：

Cho, Seung Woo ^{[1
,2
]}

Kim, Sojung ^{[1
,2
]}

Kim, Jong Min ^{[1
,2
]}

Kim, Jin-Soo ^{[1
,2
]}

机构：

[1] Seoul Natl Univ, Natl Creat Res Initiat Ctr Genome Engn, Seoul, South Korea

[2] Seoul Natl Univ, Dept Chem, Seoul, South Korea

来源：

NATURE BIOTECHNOLOGY | 2013年 / 31卷 / 03期

基金：

新加坡国家研究基金会;

关键词：

ZINC-FINGER NUCLEASES;

D O I：

10.1038/nbt.2507

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.

引用

页码：230 / 232

页数：3

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