Contemporary approaches for modifying the mouse genome

被引：27

作者：

Adams, David J. ^{[1
]}

van der Weyden, Louise ^{[1
]}

机构：

[1] Wellcome Trust Sanger Inst, Hinxton CB10 1SA, Cambs, England

来源：

PHYSIOLOGICAL GENOMICS | 2008年 / 34卷 / 03期

基金：

英国惠康基金;

关键词：

gene targeting; transposons; mutagenesis; embryonic stem cells;

D O I：

10.1152/physiolgenomics.90242.2008

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Adams DJ, van der Weyden L. Contemporary approaches for modifying the mouse genome. Physiol Genomics 34: 225-238, 2008. First published June 17, 2008; doi:10.1152/physiolgenomics.90242.2008.-The mouse is a premiere experimental organism that has contributed significantly to our understanding of vertebrate biology. Manipulation of the mouse genome via embryonic stem (ES) cell technology makes it possible to engineer an almost limitless repertoire of mutations to model human disease and assess gene function. In this review we outline recent advances in mouse experimental genetics and provide a "how-to" guide for those people wishing to access this technology. We also discuss new technologies, such as transposon-mediated mutagenesis, and resources of targeting vectors and ES cells, which are likely to dramatically accelerate the pace with which we can assess gene function in vivo, and the progress of forward and reverse genetic screens in mice.

引用

页码：225 / 238

页数：14

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