Translational repression of human matrix metalloproteinases-13 by an alternatively spliced form of T-cell-restricted intracellular antigen-related protein (TIAR)
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作者:
Yu, Q
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机构:Washington Univ, Sch Med, Dept Med & Mol Biol, St Louis, MO 63110 USA
Yu, Q
Cok, SJ
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机构:Washington Univ, Sch Med, Dept Med & Mol Biol, St Louis, MO 63110 USA
Cok, SJ
Zeng, CB
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机构:Washington Univ, Sch Med, Dept Med & Mol Biol, St Louis, MO 63110 USA
Zeng, CB
Morrison, AR
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机构:Washington Univ, Sch Med, Dept Med & Mol Biol, St Louis, MO 63110 USA
Morrison, AR
机构:
[1] Washington Univ, Sch Med, Dept Med & Mol Biol, St Louis, MO 63110 USA
[2] Washington Univ, Sch Med, Dept Pharmacol, St Louis, MO 63110 USA
Human matrix metalloproteinases-13 (HMMP13) shows a wide substrate specificity, and its expression is limited to pathological situations such as chronic inflammation and cancer. The coding sequence for HMMP13 is 86% identical to rat matrix metalloproteinases-13 (RMMP13); however, the regulation of HMMP13 and RMMP13 protein synthesis in renal mesangial cells is strikingly different. In human cells there is a discordance between HMMP13 mRNA levels and protein expression. Following IL-1beta or TGF-beta(1) stimulation, HMMP13 mRNA levels increase significantly, whereas the protein expression is absent. This discordance is because of a species-dependent translational repression. In addition to the W-untranslated region of the matrix metalloproteinases-13 (MMP13) gene, the differential expression of an alternatively spliced transcript of the RNA-binding protein TLAR in human cell cultures is also critical for this post-transcriptional regulation. Transient expression of the 17-amino acid insert of the alternatively spliced form of TIAR reverses the HMMP13 mRNA silencing observed in human and primate species. In addition, co-transfection of the alternatively spliced form of TIAR and HMMP13 into Rat2 cells suppresses HMMP13 protein expression. Thus, we report for the first time that a species-dependent TIAR isoform plays a major role in the post-transcriptional silencing for HMMP13.
机构:
Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Mol Genet & Microbiol, Piscataway, NJ 08854 USAUniv Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Mol Genet & Microbiol, Piscataway, NJ 08854 USA
机构:Univ Calif San Diego, Dept Pharmacol, Lab Gene Regulat & Signal Transduct, La Jolla, CA 92093 USA
Chen, CY
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Gherzi, R
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机构:Univ Calif San Diego, Dept Pharmacol, Lab Gene Regulat & Signal Transduct, La Jolla, CA 92093 USA
Gherzi, R
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Ong, SE
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机构:Univ Calif San Diego, Dept Pharmacol, Lab Gene Regulat & Signal Transduct, La Jolla, CA 92093 USA
Ong, SE
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Chan, EKL
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机构:Univ Calif San Diego, Dept Pharmacol, Lab Gene Regulat & Signal Transduct, La Jolla, CA 92093 USA
Chan, EKL
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Raijmakers, R
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机构:Univ Calif San Diego, Dept Pharmacol, Lab Gene Regulat & Signal Transduct, La Jolla, CA 92093 USA
Raijmakers, R
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Pruijn, GJM
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Pruijn, GJM
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Stoecklin, G
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Moroni, C
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机构:Univ Calif San Diego, Dept Pharmacol, Lab Gene Regulat & Signal Transduct, La Jolla, CA 92093 USA
Moroni, C
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Mann, M
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机构:Univ Calif San Diego, Dept Pharmacol, Lab Gene Regulat & Signal Transduct, La Jolla, CA 92093 USA
Mann, M
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Karin, M
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Univ Calif San Diego, Dept Pharmacol, Lab Gene Regulat & Signal Transduct, La Jolla, CA 92093 USAUniv Calif San Diego, Dept Pharmacol, Lab Gene Regulat & Signal Transduct, La Jolla, CA 92093 USA
机构:
WASHINGTON UNIV, SCH MED, DEPT MED & MOLEC BIOL & PHARMACOL, ST LOUIS, MO 63110 USAWASHINGTON UNIV, SCH MED, DEPT MED & MOLEC BIOL & PHARMACOL, ST LOUIS, MO 63110 USA
DaphnaIken, D
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Morrison, AR
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WASHINGTON UNIV, SCH MED, DEPT MED & MOLEC BIOL & PHARMACOL, ST LOUIS, MO 63110 USAWASHINGTON UNIV, SCH MED, DEPT MED & MOLEC BIOL & PHARMACOL, ST LOUIS, MO 63110 USA
机构:
Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Mol Genet & Microbiol, Piscataway, NJ 08854 USAUniv Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Mol Genet & Microbiol, Piscataway, NJ 08854 USA
机构:Univ Calif San Diego, Dept Pharmacol, Lab Gene Regulat & Signal Transduct, La Jolla, CA 92093 USA
Chen, CY
;
Gherzi, R
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机构:Univ Calif San Diego, Dept Pharmacol, Lab Gene Regulat & Signal Transduct, La Jolla, CA 92093 USA
Gherzi, R
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Ong, SE
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机构:Univ Calif San Diego, Dept Pharmacol, Lab Gene Regulat & Signal Transduct, La Jolla, CA 92093 USA
Ong, SE
;
Chan, EKL
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机构:Univ Calif San Diego, Dept Pharmacol, Lab Gene Regulat & Signal Transduct, La Jolla, CA 92093 USA
Chan, EKL
;
Raijmakers, R
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机构:Univ Calif San Diego, Dept Pharmacol, Lab Gene Regulat & Signal Transduct, La Jolla, CA 92093 USA
Raijmakers, R
;
Pruijn, GJM
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机构:Univ Calif San Diego, Dept Pharmacol, Lab Gene Regulat & Signal Transduct, La Jolla, CA 92093 USA
Pruijn, GJM
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Stoecklin, G
;
Moroni, C
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机构:Univ Calif San Diego, Dept Pharmacol, Lab Gene Regulat & Signal Transduct, La Jolla, CA 92093 USA
Moroni, C
;
Mann, M
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机构:Univ Calif San Diego, Dept Pharmacol, Lab Gene Regulat & Signal Transduct, La Jolla, CA 92093 USA
Mann, M
;
Karin, M
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机构:
Univ Calif San Diego, Dept Pharmacol, Lab Gene Regulat & Signal Transduct, La Jolla, CA 92093 USAUniv Calif San Diego, Dept Pharmacol, Lab Gene Regulat & Signal Transduct, La Jolla, CA 92093 USA
机构:
WASHINGTON UNIV, SCH MED, DEPT MED & MOLEC BIOL & PHARMACOL, ST LOUIS, MO 63110 USAWASHINGTON UNIV, SCH MED, DEPT MED & MOLEC BIOL & PHARMACOL, ST LOUIS, MO 63110 USA
DaphnaIken, D
;
Morrison, AR
论文数: 0引用数: 0
h-index: 0
机构:
WASHINGTON UNIV, SCH MED, DEPT MED & MOLEC BIOL & PHARMACOL, ST LOUIS, MO 63110 USAWASHINGTON UNIV, SCH MED, DEPT MED & MOLEC BIOL & PHARMACOL, ST LOUIS, MO 63110 USA