Detection of NADH and ethanol based on catalytic activity of soluble carbon nanofiber with low overpotential

被引:195
作者
Wu, Lina
Zhang, Xueji
Ju, Huangxian [1 ]
机构
[1] Nanjing Univ, Dept Chem, MOE Key Lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China
[2] Univ S Florida, Dept Chem, Tampa, FL 33620 USA
关键词
D O I
10.1021/ac061282+
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The excellent catalytic activity of a novel carbon nanomaterial, soluble carbon nanofiber (CNF) with good dispersion and wettability, to the oxidation of dihydronicotinamide adenine dinucleotide (NADH) was described for biosensing application. The edge plane sites and oxygen-rich groups presented on the CNF surface could be partially responsible for its electrocatalytic behavior, which induced a substantial decrease by 573 mV in the overpotential of NADH oxidation reaction (compared to a bare electrode). The CNF-coated electrode thus allowed highly sensitive amperometric detection of NADH with a low limit of detection (0.11 mu M), low applied potential (+0.06 V), and minimization of surface fouling. Such ability of CNF to promote the electron transfer between NADH and the electrode suggested a new, promising biocompatible platform for development of dehydrogenase-based amperometric biosensors. With alcohol dehydrogenase (ADH) as a model, the ADH/CNF-modified electrode could be constructed by a simple casting process. The proposed biosensor showed rapid and highly sensitive amperometric response to ethanol with acceptable preparation reproducibility and excellent stability.
引用
收藏
页码:453 / 458
页数:6
相关论文
共 47 条
[11]   Reactivity of poly(anilineboronic acid) with NAD+ and NADH [J].
Deore, LA ;
Freund, MS .
CHEMISTRY OF MATERIALS, 2005, 17 (11) :2918-2923
[12]  
ELVING PJ, 1982, BIOELECTROCH BIOENER, V9, P365, DOI 10.1016/0302-4598(82)80026-3
[13]   IMAGING THE INCIPIENT ELECTROCHEMICAL OXIDATION OF HIGHLY ORIENTED PYROLYTIC-GRAPHITE [J].
GOSS, CA ;
BRUMFIELD, JC ;
IRENE, EA ;
MURRAY, RW .
ANALYTICAL CHEMISTRY, 1993, 65 (10) :1378-1389
[14]   Carbon nanofiber-based active layers for fuel cell cathodes -: preparation and characterization [J].
Hacker, V ;
Wallnöfer, E ;
Baumgartner, W ;
Schaffer, T ;
Besenhard, JO ;
Schröttner, H ;
Schmied, M .
ELECTROCHEMISTRY COMMUNICATIONS, 2005, 7 (04) :377-382
[15]   Electrochemical biosensing platforms using platinum nanoparticles and carbon nanotubes [J].
Hrapovic, S ;
Liu, YL ;
Male, KB ;
Luong, JHT .
ANALYTICAL CHEMISTRY, 2004, 76 (04) :1083-1088
[16]   ELECTROCHEMICAL STABILITY OF CATECHOLS WITH A PYRENE SIDE-CHAIN STRONGLY ADSORBED ON GRAPHITE-ELECTRODES FOR CATALYTIC-OXIDATION OF DIHYDRONICOTINAMIDE ADENINE-DINUCLEOTIDE [J].
JAEGFELDT, H ;
KUWANA, T ;
JOHANSSON, G .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1983, 105 (07) :1805-1814
[17]   CATALYTIC-OXIDATION OF REDUCED NICOTINAMIDE ADENINE-DINUCLEOTIDE BY GRAPHITE-ELECTRODES MODIFIED WITH ADSORBED AROMATICS CONTAINING CATECHOL FUNCTIONALITIES [J].
JAEGFELDT, H ;
TORSTENSSON, ABC ;
GORTON, LGO ;
JOHANSSON, G .
ANALYTICAL CHEMISTRY, 1981, 53 (13) :1979-1982
[18]  
Karyakin AA, 2004, ANAL CHEM, V76, P2004, DOI 10.1021/ac035043n
[19]   Carbon nanofiber composites for the electrodes of electrochemical capacitors [J].
Kim, SU ;
Lee, KH .
CHEMICAL PHYSICS LETTERS, 2004, 400 (1-3) :253-257
[20]   Synthesis of hydrophobic and hydrophilic graphitic carbon nanofiber polymer brushes [J].
Li, L ;
Lukehart, CM .
CHEMISTRY OF MATERIALS, 2006, 18 (01) :94-99