Bioactive protein nanoarrays on nickel oxide surfaces formed by dip-pen nanolithography

被引：94

作者：

Nam, JM

Han, SW

Lee, KB

Liu, XG

Ratner, MA

Mirkin, CA

机构：

[1] Northwestern Univ, Dept Chem, Evanston, IL 60208 USA

[2] Northwestern Univ, Inst Nanotechnol, Evanston, IL 60208 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 10期

关键词：

atomic force microscopy; nanolithography; nickel; protein arrays; proteins;

D O I：

10.1002/anie.200353203

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Ink flowing from a pen: Direct-write dippen nanolithography of His-tagged proteins (ubiquitin and thioredoxin) has been used to generate biologically active protein nanoarrays with feature sizes as small as 80 nm on nickel oxide surfaces without the need for an applied electric field. The protein molecules in this system seem to diffuse from the Ni-coated atomic force microscopy (AFM) tips to the Ni-coated substrate (see diagram).

引用

收藏

页码：1246 / 1249

页数：4

相关论文

共 24 条

[1] Immobilization of histidine-tagged proteins on nickel by electrochemical dip pen nanolithography [J].

Agarwal, G ;

Naik, RR ;

Stone, MO .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (24) :7408-7412

[2] Dip-pen nanolithography in tapping mode [J].

Agarwal, G ;

Sowards, LA ;

Naik, RR ;

Stone, MO .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (02) :580-583

[3] Human thioredoxin homodimers: Regulation by pH, role of aspartate 60, and crystal structure of the aspartate 60->asparagine mutant [J].

Andersen, JF ;

Sanders, DAR ;

Gasdaska, JR ;

Weichsel, A ;

Powis, G ;

Montfort, WR .

BIOCHEMISTRY, 1997, 36 (46) :13979-13988

[4] Using nanografting to achieve directed assembly of de novo designed metalloproteins on gold [J].

Case, MA ;

McLendon, GL ;

Hu, Y ;

Vanderlick, TK ;

Scoles, G .

NANO LETTERS, 2003, 3 (04) :425-429

[5] BIOASSAY FOR TRANS-ACTIVATION USING PURIFIED HUMAN IMMUNODEFICIENCY VIRUS TAT-ENCODED PROTEIN - TRANS-ACTIVATION REQUIRES MESSENGER-RNA SYNTHESIS [J].

GENTZ, R ;

CHEN, CH ;

ROSEN, CA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (03) :821-824

[6]

Haab BB, 2001, GENOME BIOL, V2

[7] Dip-Pen nanolithography on semiconductor surfaces [J].

Ivanisevic, A ;

Mirkin, CA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (32) :7887-7889

[8] Investigation of approaches for the fabrication of protein patterns by scanning probe lithography [J].

Kenseth, JR ;

Harnisch, JA ;

Jones, VW ;

Porter, MD .

LANGMUIR, 2001, 17 (13) :4105-4112

[9] Protein nanoarrays generated by dip-pen nanolithography [J].

Lee, KB ;

Park, SJ ;

Mirkin, CA ;

Smith, JC ;

Mrksich, M .

SCIENCE, 2002, 295 (5560) :1702-1705

[10] Protein nanostructures formed via direct-write dip-pen nanolithography [J].

Lee, KB ;

Lim, JH ;

Mirkin, CA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (19) :5588-5589