Measuring amide nitrogen quadrupolar coupling by high-resolution 14N/13C NMR correlation under magic-angle spinning

被引：125

作者：

Gan, Zhehong ^{[1
]}

机构：

[1] Natl High Magnet Field Lab, Tallahassee, FL 32310 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 18期

关键词：

D O I：

10.1021/ja0578597

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The measurement of amide nitrogen 14N quadrupolar coupling by two-dimensional 14N/13C correlation experiment is presented with a natural abundant polypeptide. Directly bonded 14N/13C pairs are correlated through J and residual dipolar coupling under magic-angle spinning using a HMQC-type pulse sequence. The 14N quadrupolar coupling is measured from the isotropic second-order quadrupolar shift obtained by comparing the 14N peak positions with the 15N chemical shifts. The high spectral resolution and sensitivity through 13C detection make this method applicable to many organic, inorganic, and biological molecules for the measurement and the use of 14N quadrupolar coupling as a probe for molecular structure and dynamics. Copyright © 2006 American Chemical Society.

引用

页码：6040 / 6041

页数：2

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