Cytotoxic constituents of Amanita subjunquillea

被引：26

作者：

Kim, Ki Hyun ^{[1
]}

Choi, Sang Un ^{[2
]}

Park, Ki Moon ^{[3
]}

Seok, Soon Ja ^{[4
]}

Lee, Kang Ro ^{[1
]}

机构：

[1] Sungkyunkwan Univ, Nat Prod Lab, Coll Pharm, Suwon 440746, South Korea

[2] Korea Res Inst Chem Technol, Teajeon 305600, South Korea

[3] Sungkyunkwan Univ, Dept Food Sci & Biotechnol, Suwon 440746, South Korea

[4] RDA, Natl Inst Agr Sci & Technol, Div Appl Microbiol, Suwon 441707, South Korea

来源：

ARCHIVES OF PHARMACAL RESEARCH | 2008年 / 31卷 / 05期

关键词：

Amanita subjunquillea; ergosterol; cyclopeptide; cytotoxicity;

D O I：

10.1007/s12272-001-1196-3

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

As part of our systematic study of Korean toxic mushrooms, we have investigated the constituents of Amanita subjunquillea. The column chromatographic separation of the MeOH extract of A. subjunquillea led to the isolation of four ergosterols, two cerebrosides and four cyclopeptides. Their structures were determined by spectroscopic methods to be (22E,24R)-5 alpha,8 alpha-epidioxyergosta-6,9,22-triene-3 beta-ol (1), (22E,24R)-5 alpha,8 alpha-epidioxyergosta-6,22-dien-3 beta-ol (2), (22E,24R)-5 alpha,6 alpha-epoxyergosta-8,22-diene-3 beta,7 beta-diol (3), (24S)-ergost-7-en-3 beta-ol (4), 8,9-dihydrosoyacerebroside I (5), soyacerebroside I (6), beta-amanitin (7), phalloin (8), alpha-amanitin (9), and phalloidin (10). The compounds 1-6 and 8 were isolated for the first time from this mushroom. The isolated compounds were evaluated for the cytotoxicity against A549, SK-OV-3, SK-MEL-2 and HCT15 cells. Compound 9 exhibited significant cytotoxic activity against A549, SK-OV-3, SK-MEL-2 and HCT15 with ED50 values of 1.47, 0.26, 1.57 and 1.32 mu M, respectively.

引用

页码：579 / 586

页数：8

共 24 条

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