Detection of mutations in Mycobacterium tuberculosis genome determining resistance to fluoroquinolones by hybridization on biological microchips

被引：34

作者：

Antonova, O. V. ^{[1
,2
,3
]}

Gryadunov, D. A. ^{[1
,2
,3
]}

Lapa, S. A. ^{[1
,2
,3
]}

Kuz'min, A. V. ^{[1
,2
,3
]}

Larionova, E. E. ^{[1
,2
,3
]}

Smirnova, T. G. ^{[1
,2
,3
]}

Nosova, E. Yu. ^{[1
,2
,3
]}

Skotnikova, O. I. ^{[1
,2
,3
]}

Chernousova, L. N. ^{[1
,2
,3
]}

Moroz, A. M. ^{[1
,2
,3
]}

Zasedatelev, A. S. ^{[1
,2
,3
]}

Mikhailovich, V. M. ^{[1
,2
,3
]}

机构：

[1] Russian Acad Sci, VA Engelhardt Mol Biol Inst, Moscow, Russia

[2] Russian Acad Med Sci, Cent Inst TB, Moscow, Russia

[3] Moscow Res & Pract Ctr TB Control, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2008年 / 145卷 / 01期

关键词：

Mycobacterium tuberculosis; biochip; fluoroquinolones; hybridization; polymerase chain reaction;

D O I：

10.1007/s10517-008-0034-5

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 [基础医学];

摘要：

We developed a method of identification of Mycobacterium tuberculosis with simultaneous evaluation of the sensitivity to fluoroquinolones on a biological microchip array. The method of multiplex two-staged PCR followed by hybridization of a biochip makes it possible to detect 8 mutant variants of gyrA gene occurring in fluoroquinolone-resistant strains (similar to 85% all resistant forms) within 1 day. Using this method we analyzed 107 cultures isolated from patients with tuberculosis and 78 sputum samples. Mutations in gyrA gene were detected in 48 (92%) resistant strains. Natural S95T polymorphism in gyrA gene was detected in all resistant and in 76% sensitive strains. The sensitivity and specificity of the proposed method calculated on the basis of the analysis of sputum samples (n=78) were 94 and 100%, respectively.

引用

页码：108 / 113

页数：6

共 14 条

[1]

CHARACTERIZATION OF FLUOROQUINOLONE-RESISTANT MUTANT STRAINS OF MYCOBACTERIUM-TUBERCULOSIS SELECTED IN THE LABORATORY AND ISOLATED FROM PATIENTS [J].