Evaluation of hybridisation on oligonucleotide microarrays for analysis of drug-resistant Mycobacterium tuberculosis

被引：70

作者：

Gryadunov, D

Mikhailovich, V

Lapa, S

Roudinskii, N

Donnikov, M

Pan'kov, S

Markova, O

Kuz'min, A

Chernousova, L

Skotnikova, O

Moroz, A

Zasedatelev, A

Mirzabekov, A

机构：

[1] Russian Acad Sci, VA Engelhardt Mol Biol Inst, Moscow 119991, Russia

[2] Russian Acad Med Sci, Cent TB Res Inst, Moscow, Russia

[3] Moscow Govt, Moscow AntiTB Ctr, Moscow, Russia

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2005年 / 11卷 / 07期

基金：

俄罗斯基础研究基金会;

关键词：

biochips; hybridisation; multidrug resistance; Mycobacterium tuberculosis; oligonucleotide microarrays; PCR;

D O I：

10.1111/j.1469-0691.2005.01183.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A molecular approach was developed to identify drug-resistant strains of Mycobacterium tuberculosis by means of biochips with oligonucleotides immobilised in polyacrylamide gel pads. The technique was based on multiplex PCR, followed by hybridisation on an oligonucleotide microarray, and detected >95% of rifampicin-resistant and c. 80% of isoniazid-resistant M. tuberculosis isolates within 12 h. In total, 220 drug-resistant isolates and 131 clinical samples were tested using biochips. The sensitivity and specificity of the developed method were comparable with those of standard bacteriological testing of M. tuberculosis drug resistance.

引用

页码：531 / 539

页数：9

共 31 条

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