Novel mutations in ndh in isoniazid-resistant Mycobacterium tuberculosis isolates

被引：128

作者：

Lee, ASG ^{[1
]}

Teo, ASM

Wong, SY

机构：

[1] Natl Canc Ctr, Div Med Sci, Singapore 169610, Singapore

[2] Singapore Gen Hosp, Dept Clin Res, Singapore 169608, Singapore

[3] Tan Tock Seng Hosp, Dept Infect Dis, Singapore 308433, Singapore

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2001年 / 45卷 / 07期

关键词：

D O I：

10.1128/AAC.45.7.2157-2159.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Novel mutations in NADH dehydrogenase (ndh) were detected in 8 of 84 (9.5%) isoniazid (INH)-resistant isolates (T110A [n = 1], R268H [n = 71), but not in 22 INH-susceptible isolates of Mycobacterium tuberculosis. Significantly, all eight isolates with mutations at ndh did not have mutations at katG, kasA, or the promoter regions of inhA or ahpC, except for one isolate. Mutations in ndh appear to be an additional molecular mechanism for isoniazid resistance in M. tuberculosis.

引用

页码：2157 / 2159

页数：3

共 18 条

[1] MISSENSE MUTATIONS IN THE CATALASE-PEROXIDASE GENE, KATG, ARE ASSOCIATED WITH ISONIAZID RESISTANCE IN MYCOBACTERIUM-TUBERCULOSIS [J].

HEYM, B ;

ALZARI, PM ;

HONORE, N ;

COLE, ST .

MOLECULAR MICROBIOLOGY, 1995, 15 (02) :235-245

[2] Analysis of ahpC gene mutations in isoniazid-resistant clinical isolates of Mycobacterium tuberculosis [J].

Kelley, CL ;

Rouse, DA ;

Morris, SL .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (09) :2057-2058

[3] Contribution of kasA analysis to detection of isoniazid-resistant Mycobacterium tuberculosis in Singapore [J].

Lee, ASG ;

Lim, IHK ;

Tang, LLH ;

Telenti, A ;

Wong, SY .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (08) :2087-2089

[4] Lack of clinical significance for the common arginine-to-leucine substitution at codon 463 of the katG gene in isoniazid-resistant Mycobacterium tuberculosis in Singapore [J].

Lee, ASG ;

Tang, LLH ;

Lim, IHK ;

Ling, ML ;

Tay, L ;

Wong, SY .

JOURNAL OF INFECTIOUS DISEASES, 1997, 176 (04) :1125-1126

[5] PURIFICATION AND CHARACTERIZATION OF THE MYCOBACTERIUM-SMEGMATIS CATALASE-PEROXIDASE INVOLVED IN ISONIAZID ACTIVATION [J].

MARCINKEVICIENE, JA ;

MAGLIOZZO, RS ;

BLANCHARD, JS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (38) :22290-22295

[6] High-resolution minisatellite-based typing as a portable approach to global analysis of Mycobacterium tuberculosis molecular epidemiology [J].

Mazars, E ;

Lesjean, S ;

Banuls, AL ;

Gilbert, M ;

Vincent, V ;

Gicquel, B ;

Tibayrenc, M ;

Locht, C ;

Supply, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (04) :1901-1906

[7] Inhibition of a Mycobacterium tuberculosis β-ketoacyl ACP synthase by isoniazid [J].

Mdluli, K ;

Slayden, RA ;

Zhu, YQ ;

Ramaswamy, S ;

Pan, X ;

Mead, D ;

Crane, DD ;

Musser, JM ;

Barry, CE .

SCIENCE, 1998, 280 (5369) :1607-1610

[8] NADH dehydrogenase defects confer isoniazid resistance and conditional lethality in Mycobacterium smegmatis [J].

Miesel, L ;

Weisbrod, TR ;

Marcinkeviciene, JA ;

Bittman, R ;

Jacobs, WR .

JOURNAL OF BACTERIOLOGY, 1998, 180 (09) :2459-2467

[9] Characterization of the catalase-peroxidase gene (katG) and inhA locus in isoniazid-resistant and -susceptible strains of Mycobacterium tuberculosis by automated DNA sequencing: Restricted array of mutations associated with drug resistance [J].

Musser, JM ;

Kapur, V ;

Williams, DL ;

Kreiswirth, BN ;

vanSoolingen, D ;

vanEmbden, JDA .

JOURNAL OF INFECTIOUS DISEASES, 1996, 173 (01) :196-202

[10] Genotypic analysis of Mycobacterium tuberculosis in two distinct populations using molecular beacons:: Implications for rapid susceptibility testing [J].

Piatek, AS ;

Telenti, A ;

Murray, MR ;

El-Hajj, H ;

Jacobs, WR ;

Kramer, FR ;

Alland, D .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (01) :103-110

← 1 2 →