Surface plasmon resonance phase-shift interferometry: Real-time DNA microarray hybridization analysis

被引：33

作者：

Chen, SJ ^{[1
]}

Su, YD

Hsiu, FM

Tsou, CY

Chen, YK

机构：

[1] Natl Cheng Kung Univ, Dept Engn Sci, Tainan 701, Taiwan

[2] Natl Cent Univ, Dept Mech Engn, Chungli 320, Taiwan

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2005年 / 10卷 / 03期

关键词：

surface plasmon resonance; imaging system; microarray; DNA hybridization; phase-shifting interferometry;

D O I：

10.1117/1.1924713

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Surface plasmon resonance (SPR) phase-shift interferometry (PSI) is a novel technique which combines SPR and modified Mach-Zehnder PSI to measure the spatial phase variation caused by biomolecular interactions upon a sensing chip. The SPR-PSI imaging system offers high resolution and high-throughout screening capabilities for microarray DNA hybridization without the need for additional labeling, and provides valuable quantitative information. The SPR-PSI imaging system has an enhanced detection limit of 2.5x10(-7) refraction index change, a long-term phase stability of pi/100 in 30 min, and a spatial phase resolution of pi/300 with 100x100 mu m(2) detection area. This study successfully demonstrates the label-free observation of 15-mer DNA microarray. (c) 2005 Society of Photo-Optical Instrumentation Engineers.

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页数：6

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