Immune complexes from vasculitis patients bind to endothelial Fc receptors independent of the allelic polymorphism of FcγRIIa

被引:14
作者
Gröger, M
Fischer, GF
Wolff, K
Petzelbauer, P
机构
[1] Univ Vienna, Sch Med, Dept Dermatol, Div Gen Dermatol, A-1090 Vienna, Austria
[2] Univ Vienna, Sch Med, Dept Blood Grp Serol, A-1090 Vienna, Austria
关键词
CD32; immunoglobulin G subtypes; leukocytoclastic vasculitis; microvessels;
D O I
10.1046/j.1523-1747.1999.00620.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Cutaneous leukocytoclastic vasculitis is characterized by the deposition of circulating immune complexes, neutrophil extravasation, and vessel destruction, but mechanisms of circulating immune complexes capture within postcapillary venules are unknown. We demonstrate that circulating immune complexes from sera of vasculitis patients bind to cultured endothelium in an Fc gamma receptor IIa-dependent fashion. In lesional skin, endothelial cells bind immunoglobulin G2 > immunoglobulin G3 and immunoglobulin G4, but not immunoglobulin G1, even before obvious neutrophil transmigration and vessel damage. As the human pc gamma receptor IIa proteins exist in two allotypes (one with a histidine at position 131, which binds immunoglobulin G1, 2, 3 and the other with an arginine at position 131, which binds immunoglobulin G1, and 3, but is unable to bind immunoglobulin G2), we expected an altered prevalence of histidine 131 forms in vasculitis patients. Sequence analysis, however, revealed an equal distribution of allotypes in patients and controls. In conclusion, circulating immune complex binding to endothelial Fc gamma receptor IIa is among the initial steps in the development of vasculitis, Although immunoglobulin G2 is the predominant subtype precipitated at endothelial surfaces, it is not required for fixing circulating immune complexes to endothelium, because patients homozygote for pc gamma receptor IIa-arginine 131 equally develop leukocytoclastic vasculitis as those bearing the Fc gamma receptor IIa-histidine 131 allele. As immunoglobulin G1 is virtually absent in leukocytoclastic vasculitis lesions and immunoglobulin G4 does not bind to both Fc gamma receptor IIa alleles, these complexes, in addition to immunoglobulin G2, should contain immunoglobulin G3 in order to fix to vascular pc gamma receptor IIa, at least in persons homozygous for Fc gamma receptor IIa-arginine 131.
引用
收藏
页码:56 / 60
页数:5
相关论文
共 19 条
  • [11] HECA-452(+) T cells migrate through superficial vascular plexus but not through deep vascular plexus endothelium
    Kunstfeld, R
    Lechleitner, S
    Groger, M
    Wolff, K
    Petzelbauer, P
    [J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1997, 108 (03) : 343 - 348
  • [12] Functional separation of pseudopod extension and particle internalization during Fcγ receptor-mediated phagocytosis
    Lowry, MB
    Duchemin, AM
    Robinson, JM
    Anderson, CL
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1998, 187 (02) : 161 - 176
  • [13] Matsumura T, 1997, J IMMUNOL, V158, P3408
  • [15] A SIMPLE SALTING OUT PROCEDURE FOR EXTRACTING DNA FROM HUMAN NUCLEATED CELLS
    MILLER, SA
    DYKES, DD
    POLESKY, HF
    [J]. NUCLEIC ACIDS RESEARCH, 1988, 16 (03) : 1215 - 1215
  • [16] ON THE INTERACTION OF IGG SUBCLASSES WITH THE LOW AFFINITY FC-GAMMA-RIIA (CD32) ON HUMAN MONOCYTES, NEUTROPHILS, AND PLATELETS - ANALYSIS OF A FUNCTIONAL POLYMORPHISM TO HUMAN IGG2
    PARREN, PWHI
    WARMERDAM, PAM
    BOEIJE, LCM
    ARTS, J
    WESTERDAAL, NAC
    VLUG, A
    CAPEL, PJA
    AARDEN, LA
    VANDEWINKEL, JGJ
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1992, 90 (04) : 1537 - 1546
  • [17] FC-RECEPTORS - RUBOR REDUX
    RAVETCH, JV
    [J]. CELL, 1994, 78 (04) : 553 - 560
  • [18] HUMAN-IMMUNOGLOBULIN G (IGG) FC-RECEPTOR-IIA (CD32) POLYMORPHISM AND IGG2-MEDIATED BACTERIAL PHAGOCYTOSIS BY NEUTROPHILS
    SANDERS, LAM
    FELDMAN, RG
    VOORHORSTOGINK, MM
    DEHAAS, M
    RIJKERS, GT
    CAPEL, PJA
    ZEGERS, BJM
    VANDEWINKEL, JGJ
    [J]. INFECTION AND IMMUNITY, 1995, 63 (01) : 73 - 81
  • [19] FC-RECEPTORS INITIATE THE ARTHUS REACTION - REDEFINING THE INFLAMMATORY CASCADE
    SYLVESTRE, DL
    RAVETCH, JV
    [J]. SCIENCE, 1994, 265 (5175) : 1095 - 1098