Modelled and measured dynamics of viruses in Arctic winter sea-ice brines

被引:54
作者
Wells, Llyd E. [1 ]
Deming, Jody W. [1 ]
机构
[1] Univ Washington, Sch Oceanog, Seattle, WA 98195 USA
关键词
D O I
10.1111/j.1462-2920.2006.00984.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We describe a model based on diffusion theory and the temperature-dependent mechanism of brine concentration in sea ice to argue that, if viruses partition with bacteria into sea-ice brine inclusions, contact rates between the two can be higher in winter sea ice than in seawater, increasing the probability of infection and possible virus production. To examine this hypothesis, we determined viral and bacterial concentrations in select winter sea-ice horizons using epifluorescence microscopy. Viral concentrations ranged from 1.6 to 82 x 10(6) Ml(-1) of brine volume of the ice, with highest values in brines from coldest (-24 to -31 degrees C) ice horizons. Calculated virus-bacteria contact rates in underlying -1 degrees C seawater were similar to those in brines of -11 degrees C ice but up to 600 times lower than those in ice brines at or below -24 degrees C. We then incubated native bacterial and viral assemblages from winter sea ice for 8 days in brine at a temperature (-12 degrees C) and salinity (similar to 160 psu) near expected in situ values, monitoring their concentrations microscopically. While different cores yielded different results, consistent with known spatial heterogeneity in sea ice, these experiments provided unambiguous evidence for viral persistence and production, as well as for bacterial growth, in -12 degrees C brine.
引用
收藏
页码:1115 / 1121
页数:7
相关论文
共 26 条
[11]   Bacterial activity at-2 to-20°C in Arctic wintertime sea ice [J].
Junge, K ;
Eicken, H ;
Deming, JW .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (01) :550-557
[12]   Phylogenetic diversity of numerically important arctic sea-ice bacteria cultured at subzero temperature [J].
Junge, K ;
Imhoff, F ;
Staley, T ;
Deming, JW .
MICROBIAL ECOLOGY, 2002, 43 (03) :315-328
[13]   A microscopic approach to investigate bacteria under in situ conditions in sea-ice samples [J].
Junge, K ;
Krembs, C ;
Deming, J ;
Stierle, A ;
Eicken, H .
ANNALS OF GLACIOLOGY, VOL 33, 2001, 33 :304-310
[14]   VIRAL AND BACTERIAL DYNAMICS IN ARCTIC SEA-ICE DURING THE SPRING ALGAL BLOOM NEAR RESOLUTE, NWT, CANADA [J].
MARANGER, R ;
BIRD, DF ;
JUNIPER, SK .
MARINE ECOLOGY PROGRESS SERIES, 1994, 111 (1-2) :121-127
[15]   Virus-induced transfer of organic carbon between marine bacteria in a model community [J].
MIddelboe, M ;
Riemann, L ;
Steward, GF ;
Hansen, V ;
Nybroe, O .
AQUATIC MICROBIAL ECOLOGY, 2003, 33 (01) :1-10
[16]   Viral and bacterial production in the North Water: in situ measurements, batch-culture experiments and characterization and distribution of a virus-host system [J].
Middelboe, M ;
Nielsen, TG ;
Bjornsen, PK .
DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY, 2002, 49 (22-23) :5063-5079
[17]   VIRAL DYNAMICS - A MODEL OF THE EFFECTS OF SIZE, SHAPE, MOTION AND ABUNDANCE OF SINGLE-CELLED PLANKTONIC ORGANISMS AND OTHER PARTICLES [J].
MURRAY, AG ;
JACKSON, GA .
MARINE ECOLOGY PROGRESS SERIES, 1992, 89 (2-3) :103-116
[18]   Use of SYBR Green I for rapid epifluorescence counts of marine viruses and bacteria [J].
Noble, RT ;
Fuhrman, JA .
AQUATIC MICROBIAL ECOLOGY, 1998, 14 (02) :113-118
[19]   Virus decay and its causes in coastal waters [J].
Noble, RT ;
Fuhrman, JA .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (01) :77-83
[20]   Temperature dependence of metabolic rates for microbial growth, maintenance, and survival [J].
Price, PB ;
Sowers, T .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (13) :4631-4636