Sulfur based self-assembled monolayers (SAM's) on piezoelectric crystals for immunosensor development

被引：55

作者：

Vaughan, RD ^{[1
]}

O'Sullivan, CK ^{[1
]}

Guilbault, GG ^{[1
]}

机构：

[1] Natl Univ Ireland Univ Coll Cork, Dept Chem, Lab Sensor Dev, Cork, Ireland

来源：

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1999年 / 364卷 / 1-2期

关键词：

D O I：

10.1007/s002160051300

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Self-assembled monolayers (SAM's) of different thiols and sulfides with various structures were tested. They were investigated as a method to covalently bind antibodies to the surface of piezoelectric crystals. A capture assay using a Mouse IgG test system was performed. All tests were performed in solution. The reduction in frequency observed as binding occurred was monitored in real time. The SAM's were activated with 1-ethyl-3-[3-(dimethylamino)propyl] carbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide (NHS) prior to antibody immobilisation. Ethanolamine was used to block any available activated sizes after antibody immobilisation. The potential of this technique for immunosensor development is demonstrated.

引用

页码：54 / 57

页数：4

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