Resistance to Botrytis cinerea in scented geranium transformed with a gene encoding the antimicrobial protein Ace-AMP1

被引：65

作者：

Bi, YM

Cammue, BPA

Goodwin, PH

KrishnaRaj, S

Saxena, PK ^{[1
]}

机构：

[1] Univ Guelph, Dept Hort Sci, Guelph, ON N1G 2W1, Canada

[2] Katholieke Univ Leuven, FA Janssens Lab Genet, B-3001 Heverlee, Belgium

[3] Univ Guelph, Dept Environm Biol, Guelph, ON N1G 2W1, Canada

来源：

PLANT CELL REPORTS | 1999年 / 18卷 / 10期

关键词：

geranium; genetic transformation; Ace-AMP1; Botrytis cinerea; disease resistance;

D O I：

10.1007/s002990050670

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Scented geranium (Pelargonium sp. 'Frensham') was transformed with a gene encoding an antimicrobial protein (Ace-AMP1) from onion through an Agrobacterium-mediated transformation system. The binary vector pFAJ3033 contained the coding region of the Ace-AMP1 preproprotein-encoding cDNA. Transformants were verified by polymerase chain reaction and Southern blot analysis. Transgenic plants expressing high levels of Ace-AMP1 were identified by immunoblots and those plants were shown to have increased resistance to Botrytis cinerea leaf infection.

引用

页码：835 / 840

页数：6

共 27 条

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