Characterization and regulation of the bovine stearoyl-CoA desaturase gene promoter

被引：46

作者：

Keating, AF

Kennelly, JJ

Zhao, FQ ^{[1
]}

机构：

[1] Univ Vermont, Dept Anim Sci, Burlington, VT 05405 USA

[2] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2E1, Canada

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 344卷 / 01期

关键词：

stearoyl CoA desaturase; conjugated linoleic acid; polyunsaturated fatty acids; transcriptional regulation;

D O I：

10.1016/j.bbrc.2006.03.133

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The bovine stearoyl-CoA desaturase (Scd) gene plays an important role in the bovine mammary gland where substrates such its stearic and vaccenic acids are converted to oleic acid and conjugated linoleic acid (CLA), respectively. Up to 90% of the CLA in bovine milk is formed due to the action of this enzyme in the mammary gland. The areas of the bovine promoter of importance in regulating this key enzyme were examined and an area of 36 bp in length was identified as having a critical role in transcriptional activation and is designated the Sed transcriptional enhancer element (STE). Electrophoretic mobility shift assay detected three binding complexes on this area in Mac-T cell nuclear extracts. Treatment of cells with CLA caused a significant reduction in transcriptional activity, with this effect being mediated through the STE region. The bovine Scd gene promoter was up-regulated by insulin and down-regulated by oleic acid. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：233 / 240

页数：8

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[1] Milk fat synthesis in dairy cows is progressively reduced by increasing supplemental amounts of trans-10, cis-12 conjugated linoleic acid (CLA) [J].