Localized cell stimulation by nitric oxide using a photoactive porous coordination polymer platform

被引：122

作者：

Diring, Stephane ^{[1
]}

Wang, Dan Ohtan ^{[1
]}

Kim, Chiwon ^{[2
]}

Kondo, Mio ^{[1
]}

Chen, Yong ^{[1
]}

Kitagawa, Susumu ^{[1
,2
]}

Kamei, Ken-ichiro ^{[1
]}

Furukawa, Shuhei ^{[1
]}

机构：

[1] Kyoto Univ, Inst Integrated Cell Mat Sci WPI iCeMS, Sakyo Ku, Kyoto 6068501, Japan

[2] Kyoto Univ, Grad Sch Engn, Dept Synthet Chem & Biol Chem, Nishikyo Ku, Kyoto 6158510, Japan

来源：

NATURE COMMUNICATIONS | 2013年 / 4卷

基金：

日本科学技术振兴机构;

关键词：

METAL-ORGANIC FRAMEWORKS; CRYSTAL; GROWTH;

D O I：

10.1038/ncomms3684

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Functional cellular substrates for localized cell stimulation by small molecules provide an opportunity to control and monitor cell signalling networks chemically in time and space. However, despite improvements in the controlled delivery of bioactive compounds, the precise localization of gaseous biomolecules at the single-cell level remains challenging. Here we target nitric oxide, a crucial signalling molecule with site-specific and concentration-dependent activities, and we report a synthetic strategy for developing spatiotemporally controllable nitric oxide-releasing platforms based on photoactive porous coordination polymers. By organizing molecules with poor reactivity into polymer structures, we observe increased photoreactivity and adjustable release using light irradiation. We embed photoactive polymer crystals in a biocompatible matrix and achieve precisely controlled nitric oxide delivery at the cellular level via localized two-photon laser activation. The biological relevance of the exogenous nitric oxide produced by this strategy is evidenced by an intracellular change in calcium concentration, mediated by nitric oxide-responsive plasma membrane channel proteins.

引用

页数：8

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