The human T-type amino acid transporter-1: Characterization, gene organization, and chromosomal location

被引:104
作者
Kim, DK
Kanai, Y
Matsuo, H
Kim, JY
Chairoungdua, A
Kobayashi, Y
Enomoto, A
Cha, SH
Goya, T
Endou, H
机构
[1] Kyorin Univ, Sch Med, Dept Pharmacol & Toxicol, Tokyo 1818611, Japan
[2] Kyorin Univ, Sch Med, Dept Surg 2, Tokyo 1818611, Japan
[3] Natl Def Med Coll, Dept Physiol 1, Tokorozawa, Saitama 3598513, Japan
[4] Nagoya Univ, Sch Med, Dept Clin Prevent Med, Showa Ku, Nagoya, Aichi 4668550, Japan
[5] Japan Sci & Technol Corp, PRESTO, JST, Tokyo 1818611, Japan
基金
日本学术振兴会;
关键词
D O I
10.1006/geno.2001.6678
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
System T is a Na+-independent transport system that selectively transports aromatic amino acids. Here, we determined the structure of the human T-type amino-acid transporter-1 (TAT1) cDNA and gene (SLC16A10). The human TAT1 cDNA encoded a 515-amino-acid protein with 12 putative membrane-spanning domains. Human SLC16A10 was localized on human chromosome 6, mapped to 6q21-q22. SLC16A10 contains six exons spanning 136 kb. In contrast to rat TAT1, which is mainly present in the intestine, human TAT1 was strongly expressed in human kidney as well as in human intestine. Expression of human TAT1 in Xenopus laevis oocytes demonstrated the Na+-independent transport of tryptophan, tyrosine, phenylalanine, and L-dopa, indicating that human TAT1 is a transporter subserving system T. Because human TAT1 is proposed to be crucial to the efficient absorption of aromatic amino acids from intestine and kidney, its defect could be involved in the disruption of aromatic amino-acid transport, such as in blue diaper syndrome.
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收藏
页码:95 / 103
页数:9
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