Direct genotyping of animal and human isolates of Toxoplasma gondii from Colombia (South America)

被引：55

作者：

Gallego, C

Saavedra-Matiz, C

Gómez-Marín, JE

机构：

[1] Univ Quindio, GEPAMOL, Ctr Invest Biomed, Fac Ciencias Salud, Armenia, Colombia

[2] New York State Dept Hlth, Wadsworth Ctr, Albany, NY USA

来源：

ACTA TROPICA | 2006年 / 97卷 / 02期

关键词：

toxoplasma; genotyping; Colombia; strains; congenital toxoplasmosis;

D O I：

10.1016/j.actatropica.2005.10.001

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Genetic analysis of the SAG2 locus was performed to determine the prevalence of the main genotypes of Toxoplasina gondii (SAG2 types I, II, and III) associated with humans, cats, birds and guinea pig toxoplasmosis in Colombia. This typing was directly performed on clinical samples and autopsy material from human or animals. A total of 50 from 146 samples were positive by specific B1 Toxoplasina PCR assay and then were analyzed by restriction fragment length polymorphism in PCR-amplified SAG2 products. Characterization of the SAG2 gene was successful in 33 (66%) of the samples. Genotyping indicated that 31 (93.9%) were SAG2 type I, 1 was SAG2 type III and 1 was atypical. In birds and cats all samples were SAG2 type I. Results support a predominance of the Toxoplasma SAG2 type I circulating in human and animals in South America. (c) 2005 Elsevier B.V. All rights reserved.

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收藏

页码：161 / 167

页数：7

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