Signaling of transcriptional chemistry in the on-line detection format

被引:12
作者
Jayarajah, CN [1 ]
Thompson, M [1 ]
机构
[1] Univ Toronto, Dept Chem, Toronto, ON M5S 3H6, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
transcription; surface plasmon resonance; fluorescence; acoustic wave sensor;
D O I
10.1016/S0956-5663(01)00278-0
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A critical analysis of optical and acoustic wave instrumentation for examining the transcription apparatus and its regulation is given in the present review. The physico-chemical parameters derived from such in vitro experiments are important from a biophysical standpoint. The overall mechanism of transcription is composed of several mechanisms such as DNA-binding and promoter selection, closed and open polymerase complex formation, initiation or RNA synthesis, elongation and termination. Surface plasmon resonance (SPR) and fluorescence spectroscopy are widely employed techniques for investigating these mechanisms in real time. Although the binding of nucleotides, transcription factors (TFs) and inhibitors to RNA polymerase (RNAP) and the DNA template have been studied extensively, the synthesis of mRNA has not been investigated in detail except by methods based on electrophoresis. The use of acoustic wave physics for investigating transcriptional chemistry offers not only a time-course analysis but also the potential to gain insight into structural changes that occur during the process. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:159 / 171
页数:13
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