Megalin in normal tissues and carcinoma cells carries oligo/poly α2,8 deaminoneuraminic acid as a unique posttranslational modification

被引：12

作者：

Ziak, M ^{[1
]}

Meier, M ^{[1
]}

Roth, J ^{[1
]}

机构：

[1] Univ Zurich, Dept Pathol, Div Cell & Mol Pathol, CH-8091 Zurich, Switzerland

来源：

GLYCOCONJUGATE JOURNAL | 1999年 / 16卷 / 03期

关键词：

oligo/poly alpha 2; 8 deaminoneuraminic acid; megalin; lung; brain; placenta; F9; cells; L2;

D O I：

10.1023/A:1007068102436

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In rat kidney, megalin, a member of the low density lipoprotein receptor gene family, is the sole glycoprotein which carries oligo/poly alpha 2,8 deaminoneuraminic acid (KDN) as a posttranslational modification. We have investigated immunoprecipitated megalin from rat brain, lung and placenta, mouse yolk sac carcinoma and megalin synthesizing carcinoma cell lines, for presence of this unique glycan structure. Our immunoblot analysis revealed the presence of oligo/poly alpha 2,8 KDN on megalin in all the studied normal tissues and carcinoma cells. Furthermore, it is demonstrated that to be part of oligosaccharides O-glycosidically linked to megalin.

引用

页码：185 / 188

页数：4

共 19 条

[1] RENAL TUBULE GP330 IS A CALCIUM-BINDING RECEPTOR FOR ENDOCYTIC UPTAKE OF PROTEIN [J].

CHRISTENSEN, EI ;

GLIEMANN, J ;

MOESTRUP, SK .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1992, 40 (10) :1481-1490

[2]

CZEKAY RP, 1995, J CELL SCI, V108, P1433

[3]

FARQUHAR MG, 1995, J AM SOC NEPHROL, V6, P35

[4] Unconventional antigen retrieval for carbohydrate and protein antigens [J].

Guhl, B ;

Ziak, M ;

Roth, J .

HISTOCHEMISTRY AND CELL BIOLOGY, 1998, 110 (06) :603-611

[5] FLUOROMETRIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF N-ACETYLNEURAMINIC AND N-GLYCOLYLNEURAMINIC ACIDS AND ITS APPLICATION TO THEIR MICRODETERMINATION IN HUMAN AND ANIMAL SERA, GLYCOPROTEINS, AND GLYCOLIPIDS [J].

HARA, S ;

TAKEMORI, Y ;

YAMAGUCHI, M ;

NAKAMURA, M ;

OHKURA, Y .

ANALYTICAL BIOCHEMISTRY, 1987, 164 (01) :138-145

[6] Identification of 2-keto-3-deoxy-D-glycero-D-galactonononic acid (KDN, deaminoneuraminic acid) residues in mammalian tissues and human lung carcinoma cells - Chemical evidence of the occurrence of KDN glycoconjugates in mammals [J].

Inoue, S ;

Kitajima, K ;

Inoue, Y .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (40) :24341-24344

[7] Identification of free deaminated sialic acid (2-keto-3-deoxy-D-glycero-D-galacto-nononic acid) in human red blood cells and its elevated expression in fetal cord red blood cells and ovarian cancer cells [J].

Inoue, S ;

Lin, SL ;

Chang, TN ;

Wu, SH ;

Yao, CW ;

Chu, TY ;

Troy, FA ;

Inoue, Y .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (42) :27199-27204

[8] MONOCLONAL-ANTIBODY SPECIFIC FOR ALPHA-2-]8-LINKED OLIGO DEAMINATED NEURAMINIC ACID (KDN) SEQUENCES IN GLYCOPROTEINS - PREPARATION AND CHARACTERIZATION OF A MONOCLONAL-ANTIBODY AND ITS APPLICATION IN IMMUNOHISTOCHEMISTRY [J].

KANAMORI, A ;

INOUE, S ;

XULEI, Z ;

ZUBER, C ;

ROTH, J ;

KITAJIMA, K ;

YE, J ;

TROY, FA ;

INOUE, Y .

HISTOCHEMISTRY, 1994, 101 (05) :333-340

[9]

LUNDSTROM M, 1993, AM J PATHOL, V143, P1423

[10] BINDING AND ENDOCYTOSIS OF PROTEINS MEDIATED BY EPITHELIAL GP330 [J].

MOESTRUP, SK ;

CHRISTENSEN, EI ;

NIELSEN, S ;

JORGENSEN, KE ;

BJORN, SE ;

ROIGAARD, H ;

GLIEMANN, J .

BIOLOGY OF ALPHA2-MACROGLOBULIN, ITS RECEPTOR, AND RELATED PROTEINS, 1994, 737 :124-137

← 1 2 →