Megalin in normal tissues and carcinoma cells carries oligo/poly α2,8 deaminoneuraminic acid as a unique posttranslational modification

被引：12

作者：

Ziak, M ^{[1
]}

Meier, M ^{[1
]}

Roth, J ^{[1
]}

机构：

[1] Univ Zurich, Dept Pathol, Div Cell & Mol Pathol, CH-8091 Zurich, Switzerland

来源：

GLYCOCONJUGATE JOURNAL | 1999年 / 16卷 / 03期

关键词：

oligo/poly alpha 2; 8 deaminoneuraminic acid; megalin; lung; brain; placenta; F9; cells; L2;

D O I：

10.1023/A:1007068102436

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In rat kidney, megalin, a member of the low density lipoprotein receptor gene family, is the sole glycoprotein which carries oligo/poly alpha 2,8 deaminoneuraminic acid (KDN) as a posttranslational modification. We have investigated immunoprecipitated megalin from rat brain, lung and placenta, mouse yolk sac carcinoma and megalin synthesizing carcinoma cell lines, for presence of this unique glycan structure. Our immunoblot analysis revealed the presence of oligo/poly alpha 2,8 KDN on megalin in all the studied normal tissues and carcinoma cells. Furthermore, it is demonstrated that to be part of oligosaccharides O-glycosidically linked to megalin.

引用

页码：185 / 188

页数：4

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