Salmonella enterica requires apbC function for growth on tricarballylate:: Evidence of functional redundancy between apbC and iscU

被引:27
作者
Boyd, Jeffrey M. [1 ]
Lewis, Jeffrey A. [1 ]
Escalante-Semerena, Jorge C. [1 ]
Downs, Diana M. [1 ]
机构
[1] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA
关键词
D O I
10.1128/JB.00262-08
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Mutants of Salmonella enterica lacking apbC have nutritional and biochemical properties indicative of defects in [Fe-S] cluster metabolism. Here we show that apbC is required for S. enterica to use tricarballylate as a carbon and energy source. Tricarballylate catabolism requires three gene products, TcuA, TcuB, and TcuC. Of relevance to this work is the TcuB protein, which has two [4Fe-4S] clusters required for function, making it a logical target for the apbC effect. TcuB activity was 100-fold lower in an apbC mutant than in the isogenic apbC(+) strain. Genetic data show that derepression of the iscRSUA-hscAB-fdx-orj3 operon or overexpression of iscU from a plasmid compensates for the lack of ApbC during growth on tricarballylate. The studies described herein provide evidence that the scaffold protein IscU has a functional overlap with ApbC and that ApbC function is involved in the synthesis of active TcuB.
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页码:4596 / 4602
页数:7
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