A unique optical arrangement for obtaining spectrally resolved confocal images

被引：13

作者：

Stimson, MJ ^{[1
]}

Haralampus-Grynaviski, N ^{[1
]}

Simon, JD ^{[1
]}

机构：

[1] Duke Univ, Dept Chem, Durham, NC 27708 USA

来源：

REVIEW OF SCIENTIFIC INSTRUMENTS | 1999年 / 70卷 / 08期

关键词：

D O I：

10.1063/1.1149918

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

The design for a confocal beam-scanning microscope with a unique optical configuration for the rapid acquisition of spectrally resolved images is presented. The novel aspect of the optical configuration is the location of the detection device, which is placed in an intermediate position between fully descanned detection and nondescanned detection (direct projection). This placement allows for the practical implementation of spectrally resolved confocal imaging. The device is demonstrated by the spectral resolution of a sample of fluorescently labeled microbeads. (C) 1999 American Institute of Physics. [S0034-6748(99)03308-0].

引用

页码：3351 / 3354

页数：4

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[3]

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