A mitochondria-based method for the determination of antioxidant activities using 2′,7′-dichlorofluorescin diacetate oxidation

被引：7

作者：

He, Kai ^{[2
]}

Li, Xuegang ^{[1
]}

Ye, Xiaoli ^{[2
]}

Yuan, Lujiang ^{[1
]}

Li, Xiaoduo ^{[2
]}

Chen, Xin ^{[1
]}

Deng, Yafei ^{[1
]}

机构：

[1] Southwest Univ, Coll Pharmaceut Sci, Chem Inst Pharmaceut Resources, Chongqing 400716, Peoples R China

[2] Southwest Univ, Sch Life Sci, Chongqing 400715, Peoples R China

来源：

FOOD RESEARCH INTERNATIONAL | 2012年 / 48卷 / 02期

基金：

国家科技攻关计划;

关键词：

Antioxidant activity; DCFH-DA; Reactive oxygen species; Mitochondria-based assay; Cell-based assay; Traditional Chinese medicines; SITE-SPECIFIC DEFECTS; SUPEROXIDE-PRODUCTION; ELECTRON-TRANSPORT; PHENOLIC-COMPOUNDS; REDOX STATUS; CAPACITY; ASSAY; APOPTOSIS; STRESS; DAMAGE;

D O I：

10.1016/j.foodres.2012.04.010

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

This paper reports a physiologically relevant and rapid mitochondria-based assay for assessing antioxidant properties of pure compounds and herb extracts. The principle of this method is antioxidants can scavenge peroxyl radicals generated in mitochondria thus to inhibit 2',7'-dichlorofluorescin diacetate oxidation. Ascorbic acid, chlorogenic acid and berberine showed a dose-dependent antioxidative capacity; interestingly, lutein. Rehmannia giutinosa and Scrophularia ningpoensi exhibited pro-antioxidant properties. The antioxidant properties of herb extracts obtained by this method are in good agreement with the results measured by cell-based assay (r=0.931. p<0.001). This novel assay has acceptable accuracy, precision, reproducibility and the mitochondria-based assay may be more useful than conventional assay for detecting antioxidant capacity in a variety of foods. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：454 / 461

页数：8

共 45 条

[1]

Rapid peroxyl radical scavenging capacity (PSC) assay for assessing both hydrophilic and lipophilic antioxidants [J].