Isolation of mitochondria for biogenetical studies: An update

被引：148

作者：

Fernandez-Vizarra, Erika ^{[2
]}

Ferrin, Gustavo ^{[2
]}

Perez-Martos, Acisclo ^{[2
]}

Fernandez-Silva, Patricio ^{[2
]}

Zeviani, Massimo ^{[3
]}

Antonio Enriquez, Jose ^{[1
,2
]}

机构：

[1] Ctr Nacl Invest Cardiovasc Carlos III, Madrid 28029, Spain

[2] Univ Zaragoza, Dept Bioquim & Biol Mol & Celular, E-50009 Zaragoza, Spain

[3] Fdn IRCCS, Ist Neurol C Besta, UO Neurogenet Mol, Milan 20126, Italy

来源：

MITOCHONDRION | 2010年 / 10卷 / 03期

关键词：

Isolation of mitochondria; In organello assays; mtDNA expression studies; Protein import; TRANSCRIPTION FACTOR-A; CYTOCHROME-C-OXIDASE; TISSUE-CULTURE LINES; COMPLEX-I; RESPIRATORY-CHAIN; PHASE PARTITION; PROTEIN IMPORT; MESSENGER-RNA; DNA-SYNTHESIS; ANIMAL-CELLS;

D O I：

10.1016/j.mito.2009.12.148

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The use of good quality preparations of isolated mitochondria is necessary when studying the mitochondrial biogenetical activities. This article explains a fast and simple method for the purification of mammalian mitochondria from different tissues and cultured cells, that is suitable for the analysis of many aspects of the organelle's biogenesis. The mitochondria isolated following the protocol described here, are highly active and capable of DNA. RNA and protein synthesis. Mitochondrial tRNA aminoacylation, mtDNA-protein interactions and specific import of added proteins into the organelles, can also be studied using this kind of preparations. (C) 2009 Elsevier B.V. and Mitochondria Research Society. All rights reserved.

引用

页码：253 / 262

页数：10

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