The yeast SAS (something about silencing) protein complex contains a MYST-type putative acetyltransferase and functions with chromatin assembly factor ASF1

被引:104
作者
Osada, S
Sutton, A
Muster, N
Brown, CE
Yates, JR
Sternglanz, R
Workman, JL [1 ]
机构
[1] Penn State Univ, Dept Biochem & Mol Biol, HHMI, University Pk, PA 16802 USA
[2] Osaka Univ, Grad Sch Pharmaceut Sci, Lab Environm Biochem, Suita, Osaka 5650871, Japan
[3] SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA
[4] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
关键词
SAS; silencing; MYST; acetyl transferase; ASF1; chromatin assembly factor;
D O I
10.1101/gad.907201
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
It is well established that acetylation of histone and nonhistone proteins is intimately linked to transcriptional activation. However, loss of acetyltransferase activity has also been shown to cause silencing defects, implicating acetylation in gene silencing. The something about silencing (Sas) 2 protein of Saccharomyces cerevisiae, a member of the MYST (MOZ, Ybf2/Sas3, Sas2, and TIP60) acetyltransferase family, promotes silencing at HML and telomeres. Here we identify a similar to 450-kD SAS complex containing Sas2p, Sas4p, and the tf2f-related Sas5 protein. Mutations in the conserved acetyl-CoA binding motif of Sas2p are shown to disrupt the ability of Sas2p to mediate the silencing at HML and telomeres, providing evidence for an important role for the acetyltransferase activity of the SAS complex in silencing. Furthermore, the SAS complex is found to interact with chromatin assembly factor Asf1p, and asf1 mutants show silencing defects similar to mutants in the SAS complex. Thus, ASF1-dependent chromatin assembly may mediate the role of the SAS complex in silencing.
引用
收藏
页码:3155 / 3168
页数:14
相关论文
共 58 条
  • [21] GAS41, a highly conserved protein in eukaryotic nuclei, binds to NuMA
    Harborth, J
    Weber, K
    Osborn, M
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (41) : 31979 - 31985
  • [22] HARLOW E, 1988, ANTIBODIES ALB MANUA
  • [23] TFIIF-TAF-RNA POLYMERASE-II CONNECTION
    HENRY, NL
    CAMPBELL, AM
    FEAVER, WJ
    POON, D
    WEIL, PA
    KORNBERG, RD
    [J]. GENES & DEVELOPMENT, 1994, 8 (23) : 2868 - 2878
  • [24] Histone acetyltransferase complexes and their link to transcription
    Howe, L
    Brown, CE
    Lechner, T
    Workman, JL
    [J]. CRITICAL REVIEWS IN EUKARYOTIC GENE EXPRESSION, 1999, 9 (3-4): : 231 - 243
  • [25] Asf1 links Rad53 to control of chromatin assembly
    Hu, FH
    Alcasabas, AA
    Elledge, SJ
    [J]. GENES & DEVELOPMENT, 2001, 15 (09) : 1061 - 1066
  • [26] Histone acetyltransferase HBO1 interacts with the ORC1 subunit of the human initiator protein
    Iizuka, M
    Stillman, B
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (33) : 23027 - 23034
  • [27] Involvement of the TIP60 histone acetylase complex in DNA repair and apoptosis
    Ikura, T
    Ogryzko, VV
    Grigoriev, M
    Groisman, R
    Wang, J
    Horikoshi, M
    Scully, R
    Qin, J
    Nakatani, Y
    [J]. CELL, 2000, 102 (04) : 463 - 473
  • [28] John S, 2000, GENE DEV, V14, P1196
  • [29] Type B histone acetyltransferase Hat1p participates in telomeric silencing
    Kelly, TJ
    Qin, S
    Gottschling, DE
    Parthun, MR
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (19) : 7051 - 7058
  • [30] Le SY, 1997, YEAST, V13, P1029, DOI 10.1002/(SICI)1097-0061(19970915)13:11<1029::AID-YEA160>3.0.CO