Structure and antioxidant activity study of sulfated acetamido-polysaccharide from Radix Hedysari

被引：49

作者：

Dang, Zilong ^{[1
]}

Feng, Demei ^{[2
]}

Liu, Xiaohua ^{[1
]}

Yang, Tao ^{[1
]}

Guo, Long ^{[1
]}

Liang, Jin ^{[1
]}

Liang, Jiandi ^{[1
]}

Hu, Fangdi ^{[1
]}

Cui, Fang ^{[1
]}

Feng, Shilan ^{[1
]}

机构：

[1] Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China

[2] Second Peoples Hosp Gansu Prov, Lanzhou 730000, Peoples R China

来源：

FITOTERAPIA | 2013年 / 89卷

关键词：

Radix Hedysari; 2D NMR; Structure; Polysaccharide; Atomic force microscopy; Electron microscopy; WATER-SOLUBLE POLYSACCHARIDE; CAPSULAR POLYSACCHARIDE; NMR CHARACTERIZATION; ANTITUMOR-ACTIVITY; ELUCIDATION; PURIFICATION; METHYLATION; DERIVATIVES; CHITOSAN; FEATURES;

D O I：

10.1016/j.fitote.2013.05.011

中图分类号：

R914 [药物化学];

学科分类号：

100705 [微生物与生化药学];

摘要：

A new sulfated acetamido-heteropolysaccharide, HPS4-2A, was obtained by aqueous extraction followed by precipitation with ethanol and fractionation with DEAE column chromatography from Radix Hedysari. It was composed of rhamnose, arabinose, glucose, galactose and 2-acetamido-2-deoxy-D-galactose in the molar ratio of 10.09%:25.90%:25.90%:25.0%:12.30%. Elemental analysis indicated that HPS4-2A was a sulfated polysaccharide containing small amount of sulfate groups (1.87%). Partial acid hydrolysis, GC, GC-MS, 1D and 20 NMR spectroscopy analysis of the HPS4-2A revealed a predominance of glucose, galactose and 2-acetamido-2-deoxy-D-galactose linked in a highly-branched structure. The molecular weight of HPS4-2A was determined by HPSEC and HPSEC-MALLS.AFM study indicated that HPS4-2A took a highly branched conformation, which in consistent with the result studied by SEC-MALLS. Structural features of HPS4-2A were also investigated by SEM and TEM. Antioxidant assays demonstrated that HPS4-2A possessed of strong DPPH and hydroxyl radicals scavenging activities, suggesting that HPS4-2A could potentially be used as natural antioxidant. (c) 2013 Published by Elsevier B.V.

引用

页码：20 / 32

页数：13

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