Controlled release of DNA from carbon-paste microelectrodes

被引：34

作者：

Wang, J ^{[1
]}

Zhang, XJ ^{[1
]}

Parrado, C ^{[1
]}

Rivas, G ^{[1
]}

机构：

[1] New Mexico State Univ, Dept Chem & Biochem, Las Cruces, NM 88003 USA

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 1999年 / 1卷 / 06期

关键词：

electrochemical release; nucleic acids; DNA; microelectrodes; carbon paste; modified electrodes; gene therapy;

D O I：

10.1016/S1388-2481(99)00044-2

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Potential control has been used to release DNA from the surface of carbon electrodes under physiological pH. The protocol relies on the electrostatic adsorption of dsDNA onto a pretreated carbon-paste microelectrode, followed by an on-demand electrostatic desorption at - 1.20 V (versus Ag \ AgCl). The procedure represents a potentially attractive route for delivering the genetic material to specific locations. Various parameters influencing the release of the surface-confined DNA into the solution are explored and optimized in connection with square-wave voltammetric 'blocking' experiments, adsorptive-transfer stripping potentiometry. and energy dispersive X-ray analysis (EDX). Prospects and challenges of using DNA-modified microelectrodes for carrying therapeutic genes are discussed. (C) 1999 Elsevier Science S.A. All rights reserved.

引用

页码：197 / 202

页数：6

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