Multifunctional DNA conjugates for the in vitro selection of new catalysts

被引：13

作者：

Hausch, Felix ^{[1
]}

Jaeschke, Andres ^{[1
]}

机构：

[1] Free Univ Berlin, Fachbereich Biol Chem & Pharm, Inst Chem, D-14195 Berlin, Germany

来源：

NUCLEIC ACIDS RESEARCH | 2000年 / 28卷 / 08期

关键词：

D O I：

10.1093/nar/28.8.e35

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA-substrate conjugates are required for the direct in vitro selection of novel DNA catalysts for reactions between two small reactants. Here we describe the introduction of all necessary features into ssDNA by a novel, multifunctional primer containing a flexible PEG spacer, an o-nitrobenzyl moiety allowing for selective photocleavage, and anthracene as a reactant, a fluorescence label and/or an immobilization tag. These components were checked individually and by a mock selection.

引用

页数：3

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