Site-specific modification of enzymatically synthesized RNA: Transcription initiation and Diels-Alder reaction

被引：59

作者：

Seelig, B ^{[1
]}

Jaschke, A ^{[1
]}

机构：

[1] FREE UNIV BERLIN,INST BIOCHEM,D-14195 BERLIN,GERMANY

来源：

TETRAHEDRON LETTERS | 1997年 / 38卷 / 44期

关键词：

D O I：

10.1016/S0040-4039(97)10151-4

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Initiator nucleotides consisting of an anthracene ring system coupled to the 5'-phosphate of guanosine monophosphate via a polyethylene glycol (PEG) linker were chemically synthesized. When added to transcription reactions, these initiators were site-specifically incorporated into RNA transcripts yielding RNA conjugates with a fluorophore selectively attached to the 5'-end via a flexible linker. Besides sensitive fluorescence detection, the anthracene allowed the convenient modification with maleimides by Diels-Alder reaction. (C) 1997 Elsevier Science Ltd.

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页码：7729 / 7732

页数：4

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