Self-assembled dithiothreitol on Au surfaces for biological applications: phospholipid bilayer formation

被引：50

作者：

Creczynski-Pasa, Tania B. ^{[1
,2
]}

Daza Millone, M. Antonieta ^{[3
]}

Munford, Maximiliano L. ^{[4
]}

de Lima, Vania R. ^{[1
,2
]}

Vieira, Tiago O. ^{[1
,2
]}

Benitez, Guillermo A. ^{[3
]}

Pasa, Andre A. ^{[1
,2
]}

Salvarezza, Roberto C. ^{[3
]}

Vela, Maria E. ^{[3
]}

机构：

[1] Univ Fed Santa Catarina, Dept Ciencias Farmaceut, BR-88040900 Florianopolis, SC, Brazil

[2] Univ Fed Santa Catarina, Dept Fis, BR-88040900 Florianopolis, SC, Brazil

[3] CONICET UNLP, INIFTA, La Plata, Buenos Aires, Argentina

[4] Univ Fed Vicosa, Dept Fis, Vicosa, MG, Brazil

来源：

PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2009年 / 11卷 / 07期

关键词：

SUPPORTED LIPID-BILAYERS; SCANNING-TUNNELING-MICROSCOPY; ATOMIC-FORCE MICROSCOPY; GOLD SURFACES; ALKANETHIOL MONOLAYERS; AU(111); MEMBRANES; SULFUR; LAYERS; ATTACHMENT;

D O I：

10.1039/b811964c

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Self-assembly of dithiothreitol (DTT) on Au(111) from solution deposition has been studied by X-ray photoelectron spectroscopy and electrochemical data. DTT molecules self-assemble on Au(111) in a lying-down configuration irrespective of the concentration and temperature. XPS and electrochemical data indicate a DTT surface coverage of theta approximate to 0.16 with two S-head-Au covalent bonds per DTT molecule. The DTT monolayer turns the Au surface hydrophilic enough to allow the formation of fluid dimyristoylphosphatidylcholine (DMPC) bilayer domains by vesicle fusion as revealed by in situ atomic force imaging. Methylene blue (MB) and flavin adenine dinucleotide (FAD) have been used as probes to study molecule transport across the bilayer.

引用

页码：1077 / 1084

页数：8

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