An electrochemical biomimetic ATP-sensor

被引：14

作者：

Bücking, W

Urban, GA

Nann, T

机构：

[1] Freiburg Mat Res Ctr, FMF, D-79104 Freiburg, Germany

[2] Inst Microsyst Technol, IMTEK, D-79110 Freiburg, Germany

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2005年 / 104卷 / 01期

关键词：

ATP-sensor; biomimetic membrane; amperometric sensor; ATPase; electroanalytical chemistry;

D O I：

10.1016/j.snb.2004.04.115

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Molecular monolayers can be modified so that they mimic cell membranes. Two types of those biomimetic membranes were used in order to immobilize EF0F1 -H+-ATPase on a gold electrode. The work was motivated by the objective to measure the adenosin-triphosphate (ATP) concentrations by taking advantage of the ATPase activity. It was shown that one of the two forms of monolayers acted as a biosensor as the ATP concentration was directly proportional to the measured reduction current of the protons transported across the membrane by the ATPase. (C) 2004 Elsevier B.V. All rights reserved.

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页码：111 / 116

页数：6

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