Label-Free, All-Optical Detection, Imaging, and Tracking of a Single Protein

被引：140

作者：

Arroyo, J. Ortega ^{[1
]}

Andrecka, J. ^{[1
]}

Spillane, K. M. ^{[1
]}

Billington, N. ^{[2
]}

Takagi, Y. ^{[2
]}

Sellers, J. R. ^{[2
]}

Kukura, P. ^{[1
]}

机构：

[1] Univ Oxford, Dept Chem, Phys & Theoret Chem Lab, Oxford OX1 3QZ, England

[2] NHLBI, Lab Mol Physiol, NIH, Bethesda, MD 20892 USA

来源：

NANO LETTERS | 2014年 / 14卷 / 04期

基金：

英国工程与自然科学研究理事会; 美国国家卫生研究院;

关键词：

Single molecule detection; label-free; biosensing; myosin; 5a; interferometric scattering microscopy; HAND-OVER-HAND; MYOSIN; MOLECULES; ABSORPTION; SENSITIVITY; MICROSCOPY; POSITION; CELLS;

D O I：

10.1021/nl500234t

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Optical detection of individual proteins requires fluorescent labeling. Cavity and plasmonic methodologies enhance single molecule signatures in the absence of any labels but have struggled to demonstrate routine and quantitative single protein detection. Here, we used interferometric scattering microscopy not only to detect but also to image and nanometrically track the motion of single myosin 5a heavy meromyosin molecules without the use of labels or any nanoscopic amplification. Together with the simple experimental arrangement, an intrinsic independence from strong electronic transition dipoles and a detection limit of <60 kDa, our approach paves the way toward nonresonant, label-free sensing and imaging of nanoscopic objects down to the single protein level.

引用

页码：2065 / 2070

页数：6

共 29 条

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Single Unlabeled Protein Detection on Individual Plasmonic Nanoparticles [J].