Single Unlabeled Protein Detection on Individual Plasmonic Nanoparticles

被引：302

作者：

Ament, Irene ^{[1
]}

Prasad, Janak ^{[1
,2
]}

Henkel, Andreas ^{[1
]}

Schmachtel, Sebastian ^{[1
]}

Soennichsen, Carsten ^{[1
]}

机构：

[1] Johannes Gutenberg Univ Mainz, Inst Phys Chem, D-55128 Mainz, Germany

[2] Grad Sch Mat Sci Mainz, D-55128 Mainz, Germany

来源：

NANO LETTERS | 2012年 / 12卷 / 02期

基金：

欧洲研究理事会;

关键词：

Plasmon sensor; single molecule detection; adsorption dynamics; coverage fluctuations; LABEL-FREE; METAMATERIALS;

D O I：

10.1021/nl204496g

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

The ultimate detection limit in analytic chemistry and biology is the single molecule. Commonly, fluorescent dye labels or enzymatic amplification are employed. This requires additional labeling of the analyte, which modifies the species under investigation and therefore influences biological processes. Here, we utilize single gold nanoparticles to detect single unlabeled proteins with extremely high temporal resolution. This allows for monitoring the dynamic evolution of a single protein binding event on a millisecond time scale. The technique even resolves equilibrium coverage fluctuations, opening a window into Brownian dynamics of unlabeled macromolecules. Therefore, our method enables the study of protein folding dynamics, protein adsorption processes, and kinetics as well as nonequilibrium soft matter dynamics on the single molecule level.

引用

页码：1092 / 1095

页数：4

共 34 条

[1]

Plasmon Near-Field Coupling in Metal Dimers as a Step toward Single-Molecule Sensing [J].