Isoprenoid biosynthesis in Escherichia coli via the methylerythritol phosphate pathway:: enzymatic conversion of methylerythritol cyclodiphosphate into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol

被引：35

作者：

Seemann, M

Campos, N

Rodriguez-Concepción, M

Ibañez, E

Duvold, T

Tritsch, D

Boronat, A

Rohmer, M

机构：

[1] Univ Strasbourg 1, Inst Le Bel, CNRS, F-67070 Strasbourg, France

[2] Univ Barcelona, Fac Quim, Dept Bioquim & Biol Mol, ES-08028 Barcelona, Spain

来源：

TETRAHEDRON LETTERS | 2002年 / 43卷 / 08期

关键词：

biosynthesis; 2-C-methyl-D-erythritol; 4-phosphate; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate; (E)-2-methylbut-2-ene-1,4-diol; gcpE; isoprenoids; lytB;

D O I：

10.1016/S0040-4039(02)00003-5

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A crude cell-free system from an Escherichia coli strain overexpressing the cluster containing the three genes yfgA. yfgB. and gcpE converted 2-C-menthyl-D-erythritol 2.4-cyclodiphosphate (1) into a phosphorylated derivative of (E)-2-methylbut-2-ene-1.4-diol (6). which most probably represents a novel intermediate in the methylerythritol phosphate path a for isoprenoid biosynthesis, The free diol 6 Was accumulated by phosphatase treatment of the crude enzyme preparation and,is identified by comparison with a synthetic reference, (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

收藏

页码：1413 / 1415

页数：3

相关论文

共 15 条

[1] LytB, a novel gene of the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli [J].

Altincicek, B ;

Kollas, AK ;

Eberl, M ;

Wiesner, J ;

Sanderbrand, S ;

Hintz, M ;

Beck, E ;

Jomma, H .

FEBS LETTERS, 2001, 499 (1-2) :37-40

[2] GcpE is involved in the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli [J].

Altincicek, B ;

Kollas, AK ;

Sanderbrand, S ;

Wiesner, J ;

Hintz, M ;

Beck, E ;

Jomaa, H .

JOURNAL OF BACTERIOLOGY, 2001, 183 (08) :2411-2416

[3] Escherichia coli engineered to synthesize isopentenyl diphosphate and dimethylallyl diphosphate from mevalonate:: a novel system for the genetic analysis of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis [J].

Campos, N ;

Rodríguez-Concepción, M ;

Sauret-Güeto, S ;

Gallego, F ;

Lois, LM ;

Boronat, A .

BIOCHEMICAL JOURNAL, 2001, 353 :59-67

[4] Identification of gcpE as a novel gene of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis in Escherichia coli [J].

Campos, N ;

Rodríguez-Concepción, M ;

Seemann, M ;

Rohmer, M ;

Boronat, A .

FEBS LETTERS, 2001, 488 (03) :170-173

[5] Evidence of a role for LytB in the nonmevalonate pathway of isoprenoid biosynthesis [J].

Cunningham, FX ;

Lafond, TP ;

Gantt, E .

JOURNAL OF BACTERIOLOGY, 2000, 182 (20) :5841-5848

[6] Incorporation of 2-C-methyl-D-erythritol, a putative isoprenoid precursor in the mevalonate-independent pathway, into ubiquinone and menaquinone of Escherichia coli [J].

Duvold, T ;

Cali, P ;

Bravo, JM ;

Rohmer, M .

TETRAHEDRON LETTERS, 1997, 38 (35) :6181-6184

[7] Deoxyxylulose phosphate pathway to terpenoids [J].

Eisenreich, W ;

Rohdich, F ;

Bacher, A .

TRENDS IN PLANT SCIENCE, 2001, 6 (02) :78-84

[8] Studies on the nonmevalonate pathway to terpenes: The role of the GcpE (IspG) protein [J].

Hecht, S ;

Eisenreich, W ;

Adam, P ;

Amslinger, S ;

Kis, K ;

Bacher, A ;

Arigoni, D ;

Rohdich, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (26) :14837-14842

[9] Identification of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate as a major activator for human γδ T cells in Escherichia coli [J].

Hintz, M ;

Reichenberg, A ;

Altincicek, B ;

Bahr, U ;

Gschwind, RM ;

Kollas, AK ;

Beck, E ;

Wiesner, J ;

Eberl, M ;

Jomaa, H .

FEBS LETTERS, 2001, 509 (02) :317-322

[10]

OSTROVSKY D, 1992, BIOFACTORS, V3, P261