Autocrine and paracrine regulation of lymphocyte CB2 receptor expression by TGF-β

被引：47

作者：

Gardner, B

Zu, LX

Sharma, S

Liu, Q

Makriyannis, A

Tashkin, DP

Dubinett, SM

机构：

[1] Univ Calif Los Angeles, Sch Med, Immunol Pulm Lab, Los Angeles, CA 90073 USA

[2] Univ Calif Los Angeles, Sch Med, Div Pulm & Crit Care Med, Los Angeles, CA 90073 USA

[3] VA W Los Angeles Healthcare Ctr, Los Angeles, CA 90073 USA

[4] Univ Connecticut, Dept Pharmaceut Sci, Storrs, CT 06269 USA

[5] Univ Connecticut, Dept Mol & Cell Biol, Storrs, CT 06269 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 290卷 / 01期

关键词：

T lymphocytes; G proteins; cytokines;

D O I：

10.1006/bbrc.2001.6179

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The marijuana-derived cannabinoid Delta(9)-tetrahydrocannabinol (THC) has been shown to be immunosuppressive. We report that THC induces the immunosuppressive cytokine TGF-beta by human peripheral blood lymphocytes (PBL). The ability of THC to stimulate TGF-beta production was blocked by the CB2 receptor specific antagonist SR144528 but not by the CB1 specific antagonist AM251. Furthermore, our data suggest that TGF-beta actively regulates lymphocyte CB2 receptor expression in an autocrine and paracrine manner. Whereas the addition of recombinant TGF-beta to PBL cultures downregulated CB2 receptor expression, anti-TGF-beta antibody treatment increased CB2 receptor expression. We conclude that one mechanism by which THC contributes to immune suppression is by stimulating an enhanced production of lymphocyte TGF-beta. (C) 2002 Elsevier Science.

引用

页码：91 / 96

页数：6

共 30 条

[1] Marijuana and cocaine impair alveolar macrophage function and cytokine production [J].